Application | Comment | Organism |
---|---|---|
medicine | one of the major human fungal pathogens, expanded knowledge about essential metabloic processes and enzymes in this pathogenic fungus can pave the way for the development of more effective treatments | Candida albicans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Candida albicans | |
ATP | - |
Candida albicans | |
CaCl2 | - |
Candida albicans | |
CDP | - |
Candida albicans | |
CTP | - |
Candida albicans | |
diphosphate | most efficient inhibitor | Candida albicans | |
GTP | - |
Candida albicans | |
additional information | no substrate inhibition with 2-deoxy-CDPdiacylglycerol, CMP and to a lesser extent AMP shows low inhibition of the enzyme | Candida albicans | |
phosphate | - |
Candida albicans | |
Triton X-100 | above 1% | Candida albicans | |
TTP | - |
Candida albicans | |
UDP | - |
Candida albicans | |
UTP | - |
Candida albicans | |
ZnCl2 | - |
Candida albicans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.029 | - |
2-deoxy-CDP-diacylglycerol | pH 9.0, 37°C | Candida albicans | |
0.036 | - |
CDP-diacylglycerol | pH 9.0, 37°C | Candida albicans | |
0.55 | - |
myo-inositol | pH 9.0, 30°C | Candida albicans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | membrane-bound | Candida albicans | 16020 | - |
microsome | - |
Candida albicans | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | reaction and reverse reaction dependent on either Mg2+ or Mn2+ | Candida albicans | |
Mn2+ | - |
Candida albicans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CDP-diacylglycerol + myo-inositol | Candida albicans | - |
CMP + phosphatidyl-1D-myo-inositol | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida albicans | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.001 | 0.002 | - |
Candida albicans |
Storage Stability | Organism |
---|---|
-80°C, frozen in liquid N2 and stored, no loss of activity is detected during storage for up to 2 months | Candida albicans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-deoxy-CDP-diacylglycerol + myo-inositol | maximal activity only 36% of the optimal activity reached with CDP-diacylglycerol | Candida albicans | CMP + ? | - |
r | |
CDP-diacylglycerol + myo-inositol | - |
Candida albicans | CMP + phosphatidyl-1D-myo-inositol | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Candida albicans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 50 | thermal denaturation at higher temperatures, half-life at 50°C 30 min | Candida albicans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
highest activity in Tris buffer, only 43% of the activity in glycine/NaOH buffer at pH 9.5 | Candida albicans |
9.5 | - |
glycine NaOH buffer | Candida albicans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 10.5 | - |
Candida albicans |