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Literature summary for 2.7.8.11 extracted from

  • Klezovitch, O.; Brandenburger, Y.; Geindre, M.; Deshusses, J.
    Characterization of reactions catalysed by yeast phosphatidylinositol synthase (1993), FEBS Lett., 320, 256-260.
    View publication on PubMed
Search for more literature for 2.7.8.11

Cloned(Commentary)

Cloned (Comment) Organism
gene PIS cloned with pUC8 and expressed in Escherichia coli HB101 Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
 CMP pH 8.0, 30°C Saccharomyces cerevisiae
0.066
-
 myo-inositol pH 8.0, 30°C, CMP-independent exchange reaction Saccharomyces cerevisiae
0.09
-
 myo-inositol pH 8.0, 30°C, forward reaction Saccharomyces cerevisiae
0.277
-
 myo-inositol pH 8.0, 30°C, CMP-stimulated exchange reaction Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CDP-diacylglycerol + myo-inositol Saccharomyces cerevisiae
-
 CMP + phosphatidyl-1D-myo-inositol
-
 r

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 yeast
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CDP-diacylglycerol + myo-inositol
-
 Saccharomyces cerevisiae CMP + phosphatidyl-1D-myo-inositol
-
 r
additional information enzyme also catalyzes diacylglycerol-independent exchange reaction between phosphatidylinositol and inositol Saccharomyces cerevisiae ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 optimum for exchange reaction Saccharomyces cerevisiae
8
-
-
 Saccharomyces cerevisiae
8.5
-
 pH optimum for reverse reaction Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
6.5 10
-
 Saccharomyces cerevisiae