Crystallization (Comment) | Organism |
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cocrystal structures of the Hermes transposase bound to DNA that mimics the reaction step immediately prior to hairpin formation. A large DNA conformational change occurs between the initial cleavage step and subsequent hairpin formation that changes which strand is acted upon by a single active site. The complement of divalent metal ions bound by the catalytically essential DDE residues, and the identity of the -2 flanking base pair affect the conformationbal change. For efficient hairpin formation an A:T basepair is favored at the -2 position. The histidine residue within a conserved C/DxxH motif interacts directly with the scissile phosphate | Musca domestica |
Organism | UniProt | Comment | Textmining |
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Musca domestica | Q25438 | - |
- |
Synonyms | Comment | Organism |
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Hermes transposase | - |
Musca domestica |