Literature summary for 2.7.7.B22 extracted from

Querques, I.; Mades, A.; Zuliani, C.; Miskey, C.; Alb, M.; Grueso, E.; Machwirth, M.; Rausch, T.; Einsele, H.; Ivics, Z.; Hudecek, M.; Barabas, O.
A highly soluble Sleeping Beauty transposase improves control of gene insertion (2019), Nat. Biotechnol., 37, 1502-1512 .

Search for more literature for 2.7.7.B22

Application

Application	Comment	Organism
medicine	an hyperactive variant with enhanced solubility and stability can be delivered with transposon DNA to genetically modify cell lines and embryonic, hematopoietic and induced pluripotent stem cells. The variant is used to generate chimeric antigen receptor T-cells, which exhibit potent anti-tumor activity in vitro and in xenograft mice. The variant spontaneously penetrates cells, enabling modification of induced pluripotent stem cells and generation of generate chimeric antigen receptor T-cells without the use of transfection reagents	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	introduction of mutation I212S to variant SB100X increases solubility. Additional substitution C176S generates a remarkably high solubility. Mutations C197S, C304S and C316S compromise protein solubility	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Synonyms

Synonyms	Comment	Organism
SB100X	-	Homo sapiens
sleeping beauty transposase	-	Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
95	-	stable up to, variant SB100X carrying mutations I212S/C176S	Homo sapiens

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Release 2023.1 (January 2023)