Protein Variants | Comment | Organism |
---|---|---|
F1285A | mutation abolishes RNA capping activity | Lyssavirus rabies |
G1112A | mutation abolishes RNA capping activity | Lyssavirus rabies |
H1241A | mutation abolishes RNA capping activity | Lyssavirus rabies |
Q1286A | mutation abolishes RNA capping activity | Lyssavirus rabies |
R1242A | mutation abolishes RNA capping activity | Lyssavirus rabies |
S1155A | about 10% of wild-type activity | Lyssavirus rabies |
S1168A | about 10% of wild-type activity | Lyssavirus rabies |
T1170A | mutation abolishes RNA capping activity | Lyssavirus rabies |
W1201A | mutation abolishes RNA capping activity | Lyssavirus rabies |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lyssavirus rabies | Q9IPJ5 | - |
- |
Lyssavirus rabies Nishigahara RCEH | Q9IPJ5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-triphospho-mRNA + GDP | - |
Lyssavirus rabies | diphosphate + guanosine 5'-triphospho-mRNA | - |
? | |
5'-triphospho-mRNA + GDP | - |
Lyssavirus rabies Nishigahara RCEH | diphosphate + guanosine 5'-triphospho-mRNA | - |
? | |
additional information | rabies virus large structural protein shows guanosine 51-triphosphatase and GDP polyribonucleotidyltransferase activities. Enzyme caps 5'-triphosphorylated but not 5'-diphosphorylated RABV mRNA-start sequences, 5'-AACA(C/U), with GDP to generate the 5'-terminal cap structure G(5')ppp(5')A. The 5'-AAC sequence in the substrate RNAs is strictly essential for RNA capping with the RABV L protein | Lyssavirus rabies | ? | - |
? | |
additional information | rabies virus large structural protein shows guanosine 51-triphosphatase and GDP polyribonucleotidyltransferase activities. Enzyme caps 5'-triphosphorylated but not 5'-diphosphorylated RABV mRNA-start sequences, 5'-AACA(C/U), with GDP to generate the 5'-terminal cap structure G(5')ppp(5')A. The 5'-AAC sequence in the substrate RNAs is strictly essential for RNA capping with the RABV L protein | Lyssavirus rabies Nishigahara RCEH | ? | - |
? |