Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diphosphate | inhibits formation of the enzyme-RNa complex | vesicular stomatitis Indiana virus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | assay in presence of 1 mM divalent cation, Mn2+ is preferred over Mg2+ | vesicular stomatitis Indiana virus | |
Mn2+ | assay in presence of 1 mM divalent cation, Mn2+ is preferred over Mg2+ | vesicular stomatitis Indiana virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
vesicular stomatitis Indiana virus | P03523 | - |
- |
vesicular stomatitis Indiana virus San Juan | P03523 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-triphospho-mRNA + GDP | - |
vesicular stomatitis Indiana virus | diphosphate + guanosine 5'-triphospho-mRNA | - |
? | |
5'-triphospho-mRNA + GDP | - |
vesicular stomatitis Indiana virus San Juan | diphosphate + guanosine 5'-triphospho-mRNA | - |
? | |
additional information | a 5'-monophosphorylated viral mRNA-start sequence is transferred to GDP generated from GTP via a covalent enzyme-RNA intermediate. The enzyme uses only 5'-triphosphorylated RNA substrates. It efficiently caps RNAs containing an A(purine)CNG sequence with GDP. Especially, the first A and third pyrimidine residues appear to be essential for the RNA substrate activity. No substrates: ppApApCpApG or pppApCpGpApA | vesicular stomatitis Indiana virus | ? | - |
? | |
additional information | a 5'-monophosphorylated viral mRNA-start sequence is transferred to GDP generated from GTP via a covalent enzyme-RNA intermediate. The enzyme uses only 5'-triphosphorylated RNA substrates. It efficiently caps RNAs containing an A(purine)CNG sequence with GDP. Especially, the first A and third pyrimidine residues appear to be essential for the RNA substrate activity. No substrates: ppApApCpApG or pppApCpGpApA | vesicular stomatitis Indiana virus San Juan | ? | - |
? | |
pppApApCpApG + GDP | - |
vesicular stomatitis Indiana virus | diphosphate + guanosine 5'-pppApApCpApG | recombinant enzyme specifically caps pppApApCpApG with GDP | ? | |
pppApApCpApG + GDP | - |
vesicular stomatitis Indiana virus San Juan | diphosphate + guanosine 5'-pppApApCpApG | recombinant enzyme specifically caps pppApApCpApG with GDP | ? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | vesicular stomatitis Indiana virus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.8 | - |
assay at | vesicular stomatitis Indiana virus |