Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | stimulates ADP polymerization maximally at 10 mM, GDP polymerization maximally at 5 mM, inhibition above | Bacillus amyloliquefaciens | |
additional information | relatively insensitive to N-ethylmaleimide and high concentrations of KCl | Bacillus amyloliquefaciens | |
phosphate | complete inhibition of polymerization at low concentrations | Bacillus amyloliquefaciens | |
Poly(A) | inhibition of poly(A) synthesis | Bacillus amyloliquefaciens | |
Poly(G) | inhibition of poly(A) synthesis | Bacillus amyloliquefaciens | |
rifamycin SV | partial inhibition of polymerization | Bacillus amyloliquefaciens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Bacillus amyloliquefaciens | |
Mg2+ | maximal activation of ADP and GDP polymerization at 10 and 5 mM, respectively, inhibition at higher concentrations | Bacillus amyloliquefaciens | |
Mn2+ | can partially replace Mg2+ in activation | Bacillus amyloliquefaciens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus amyloliquefaciens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
phase partition, ammonium sulfate, agarose gel filtration, DEAE-Sephadex | Bacillus amyloliquefaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
poly(A) + ADP | - |
Bacillus amyloliquefaciens | poly(A)+1 + phosphate | - |
r | |
poly(G) + GDP | - |
Bacillus amyloliquefaciens | poly(G)+1 + phosphate | - |
r | |
ribonucleoside 5'-diphosphate + phosphate | - |
Bacillus amyloliquefaciens | ribonucleoside 5'-diphosphate + phosphate | exchange reaction | ? | |
RNAn + a nucleoside diphosphate | primer-independent activity | Bacillus amyloliquefaciens | RNAn+1 + phosphate | - |
? | |
RNAn + a nucleoside diphosphate | polymerization of GDP | Bacillus amyloliquefaciens | RNAn+1 + phosphate | - |
? | |
RNAn + a nucleoside diphosphate | polymerization of ADP | Bacillus amyloliquefaciens | RNAn+1 + phosphate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
polymerization of ADP | Bacillus amyloliquefaciens |
7 | 9 | polymerization of GDP | Bacillus amyloliquefaciens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9.5 | almost no activity below | Bacillus amyloliquefaciens |