Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.8 extracted from

  • Waters, M.F.; McLennan, A.G.; Carr, N.G.
    Purification and activity gel analysis of polynucleotide phosphorylase from the cyanobacterium Nostoc sp. MAC (1989), J. Gen. Microbiol., 135, 2045-2054.
No PubMed abstract available
Search for more literature for 2.7.7.8

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Nostoc sp.
Mn2+ 7-9% of activity with Mg2+ Nostoc sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
91000
-
 alpha3, 3 * 91000, SDS-PAGE Nostoc sp.
215000 240000 gel filtration Nostoc sp.
240000
-
 sedimentation equilibrium Nostoc sp.

Organism

Organism UniProt Comment Textmining
Nostoc sp.
-
 MAC
-
Nostoc sp. MAC
-
 MAC
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-cellulose, Sephacryl S-300, Mono Q, poly(A)-Sepharose Nostoc sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2
-
 at pH 10.5 Nostoc sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly(A) + ADP
-
 Nostoc sp. poly(A)+1 + phosphate
-
 r
poly(A) + ADP
-
 Nostoc sp. MAC poly(A)+1 + phosphate
-
 r
poly(U)+ UDP
-
 Nostoc sp. poly(U)+1 + phosphate
-
 r
RNAn + a nucleoside diphosphate primer-independent activity Nostoc sp. RNAn+1 + phosphate
-
 ?
RNAn + a nucleoside diphosphate polymerization of UDP Nostoc sp. RNAn+1 + phosphate
-
 ?
RNAn + a nucleoside diphosphate polymerization of ADP Nostoc sp. RNAn+1 + phosphate
-
 ?
RNAn + a nucleoside diphosphate purified enzyme is less dependent on a primer than the enzyme in crude extracts Nostoc sp. RNAn+1 + phosphate
-
 ?
RNAn + a nucleoside diphosphate primer-independent activity Nostoc sp. MAC RNAn+1 + phosphate
-
 ?
RNAn + a nucleoside diphosphate polymerization of UDP Nostoc sp. MAC RNAn+1 + phosphate
-
 ?
RNAn + a nucleoside diphosphate polymerization of ADP Nostoc sp. MAC RNAn+1 + phosphate
-
 ?
RNAn + a nucleoside diphosphate purified enzyme is less dependent on a primer than the enzyme in crude extracts Nostoc sp. MAC RNAn+1 + phosphate
-
 ?

Subunits

Subunits Comment Organism
trimer alpha3, 3 * 91000, SDS-PAGE Nostoc sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Nostoc sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.5
-
-
 Nostoc sp.

pH Range

pH Minimum pH Maximum Comment Organism
9.5 11.5 14% of maximal activity at pH 9.5, 70% of maximal activity at pH 11.5 Nostoc sp.