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Literature summary for 2.7.7.8 extracted from

  • Barbehenn, E.K.; Craine, J.E.; Chrambach, A.; Klee, C.B.
    Characterization of polynucleotide phosphorylase from Micrococcus luteus and isolation of the 13,000 base poly(A) product of the polymerization reaction (1982), J. Biol. Chem., 257, 1007-1016.
    View publication on PubMed
Search for more literature for 2.7.7.8

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
92000
-
 3 * 92000, SDS-PAGE, prior to purification the enzyme exists in oligomeric forms Micrococcus luteus
237000
-
 sedimentation equilibrium Micrococcus luteus

Organism

Organism UniProt Comment Textmining
Micrococcus luteus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Micrococcus luteus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly(A) + ADP
-
 Micrococcus luteus poly(A)+1 + phosphate poly(A) polymerization product containing 8000-13000 nucleotides r
RNAn + a nucleoside diphosphate polymerization of ADP Micrococcus luteus RNAn+1 + phosphate
-
 ?
RNAn + a nucleoside diphosphate primer independent enzyme Micrococcus luteus RNAn+1 + phosphate
-
 ?

Subunits

Subunits Comment Organism
trimer 3 * 92000, SDS-PAGE, prior to purification the enzyme exists in oligomeric forms Micrococcus luteus

pI Value

Organism Comment pI Value Maximum pI Value
Micrococcus luteus isoelectric focusing, minor band at pH 6.8
-
 6.1