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Literature summary for 2.7.7.8 extracted from
- Purification of polynucleotide phosphorylase by affinity chromatography and some properties of the purified enzymes (1974), Nucleic Acids Res., 1, 1763-1773.
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Geobacillus stearothermophilus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| affinity chromatography | Escherichia coli |
| polyethylene imine, Sepharose 4B, affinity chromatography on oligo(dT)-Sepharose | Geobacillus stearothermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 22.5 | - |
- |
Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| poly(A) + ADP | - |
Escherichia coli | poly(A)+1 + phosphate | - |
r |  |
| poly(A) + ADP | strong preference for ADP and poly(A) in phosphorolysis and polymerization reaction | Geobacillus stearothermophilus | poly(A)+1 + phosphate | - |
r | |
| poly(A)+1 + phosphate | - |
Escherichia coli | poly(A) + ADP | - |
r | |
| poly(A)+1 + phosphate | - |
Geobacillus stearothermophilus | poly(A) + ADP | - |
r | |
| poly(I) + IDP | - |
Escherichia coli | poly(I)+1 + phosphate | - |
? | |
| poly(I) + IDP | - |
Geobacillus stearothermophilus | poly(I)+1 + phosphate | phosphorolysis at 14% of activity with poly(A) | ? | |
| RNAn + a nucleoside diphosphate | polymerization of ADP | Escherichia coli | RNAn+1 + phosphate | - |
? | |
| RNAn + a nucleoside diphosphate | polymerization of ADP | Geobacillus stearothermophilus | RNAn+1 + phosphate | - |
? | |
| RNAn + a nucleoside diphosphate | strong preference for ADP | Geobacillus stearothermophilus | RNAn+1 + phosphate | - |
? | |
| RNAn+1 + phosphate | phosphorolysis of poly(I) | Escherichia coli | RNAn + a nucleoside diphosphate | - |
r | |
| RNAn+1 + phosphate | phosphorolysis of poly(I) | Geobacillus stearothermophilus | RNAn + a nucleoside diphosphate | - |
r | |
| RNAn+1 + phosphate | strong preference for poly(A) | Geobacillus stearothermophilus | RNAn + a nucleoside diphosphate | - |
r | |
| RNAn+1 + phosphate | phosphorolysis of poly(A) | Escherichia coli | RNAn + a nucleoside diphosphate | - |
r | |
| RNAn+1 + phosphate | phosphorolysis of poly(A) | Geobacillus stearothermophilus | RNAn + a nucleoside diphosphate | - |
r | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.2 | - |
phosphorolysis of poly(A) | Geobacillus stearothermophilus |
| 10 | - |
polymerization of ADP | Geobacillus stearothermophilus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | 9 | phosphorolysis of poly(A), approx. 25% of maximal activity at pH 7.3 and pH 9.1, respectively | Geobacillus stearothermophilus |
| 8.4 | 10 | polymerization of ADP, approx. 25% of maximal activity at pH 8.6 | Geobacillus stearothermophilus |
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