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Literature summary for 2.7.7.8 extracted from
- Purification and characterization of polynucleotide phosphorylase from Escherichia coli. Probe for the analysis of 3 sequences of RNA (1977), J. Biol. Chem., 252, 6885-6888.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000166 | - |
globin mRNA | pH 8.0, 37°C, phosphorolysis of poly(A)tail from rabbit globin mRNA | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 230000 | - |
ultracentrifugation, equilibrium sedimentaion analysis | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, DEAE-cellulose, RNA-sepharose | Escherichia coli |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 27.67 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 3 mg/ml enzyme concentration, 3 years, no loss of activity | Escherichia coli |
| -20°C, dilute solution, at least 2 months, no loss of activity | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + globin mRNAn | - |
Escherichia coli | globin mRNAn+1 + phosphate | - |
? |  |
| rabbit globin mRNAn+1 + phosphate | only the poly(A) tail of the mRNA is removed | Escherichia coli | ADP + rabbit globin mRNAn | - |
? | |
| RNAn + a nucleoside diphosphate | - |
Escherichia coli | RNAn+1 + phosphate | - |
r | |
| RNAn+1 + phosphate | processive phosphorolysis of the poly(A) tail of each globin mRNA chain | Escherichia coli | RNAn + a nucleoside diphosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | SDS-PAGE | Escherichia coli |
| trimer | alpha3, 3 * 84000-95000, enzyme form A, ultrastructural observations | Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Escherichia coli | isoelectric focusing | - |
6.1 |
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Release 2023.1 (January 2023)
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