Activating Compound | Comment | Organism | Structure |
---|---|---|---|
BeF3- | - |
Pseudomonas syringae | |
additional information | oligomerization-dependent activation of WspR, the stalks are key regulatory elements for the oligomerization, activation, and autoinhibition of WspR | Pseudomonas syringae |
Cloned (Comment) | Organism |
---|---|
the vectors pET21 and pProExHT are used | Pseudomonas syringae |
Crystallization (Comment) | Organism |
---|---|
the crystal structure of full-length WspR is determined at 2.8 A resolution, of WspGCN4-RGGDEF at 1.94 A resolution | Pseudomonas syringae |
Protein Variants | Comment | Organism |
---|---|---|
WspGCN4-RGGDEF | fusion protein, coiled-coil segment of GCN4 from Saccharomyces cerevisiae, C-terminal diguanylate cyclase domain with the characteristic GGDEF motif and the active site | Pseudomonas syringae |
WspRD70N | mutant, point mutation at the predicted phosphorylation site in the REC domain | Pseudomonas syringae |
WspRGGDEF | residues 172-347, C-terminal diguanylate cyclase domain with the characteristic GGDEF motif and the active site | Pseudomonas syringae |
WspRstalk-GGDEF | residues 140-347, catalytic activity is modulated by the helical stalk motif, C-terminal diguanylate cyclase domain with the characteristic GGDEF motif and the active site | Pseudomonas syringae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cyclic di-3',5'-guanylate | product inhibition | Pseudomonas syringae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00597 | - |
GTP | WspR full-length, cyclic di-3',5'-guanylate-free | Pseudomonas syringae | |
0.01296 | - |
GTP | WspGCN4-RGGDEF, cyclic di-3',5'-guanylate-free | Pseudomonas syringae | |
0.01307 | - |
GTP | WspGCN4-RGGDEF, cyclic di-3',5'-guanylate-bound | Pseudomonas syringae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP | Pseudomonas syringae | - |
diphosphate + cyclic di-3',5'-guanylate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas syringae | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | - |
Pseudomonas syringae |
Purification (Comment) | Organism |
---|---|
by Ni-NTA affinity chromatography, furthermore a Superdex 200 and a gel filtration column are used | Pseudomonas syringae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP | - |
Pseudomonas syringae | diphosphate + cyclic di-3',5'-guanylate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | - |
Pseudomonas syringae |
Synonyms | Comment | Organism |
---|---|---|
diguanylate cyclase | - |
Pseudomonas syringae |
WspR | - |
Pseudomonas syringae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.5 | - |
GTP | WspR full-length, cyclic di-3',5'-guanylate-free | Pseudomonas syringae | |
40.56 | - |
GTP | WspGCN4-RGGDEF, cyclic di-3',5'-guanylate-bound | Pseudomonas syringae | |
59.05 | - |
GTP | WspGCN4-RGGDEF, cyclic di-3',5'-guanylate-free | Pseudomonas syringae |