Activating Compound | Comment | Organism | Structure |
---|---|---|---|
O2 | - |
Desulfotalea psychrophila |
Cloned (Comment) | Organism |
---|---|
into the vector pET3a for expression in Escherichia coli BL21DE3 cells | Desulfotalea psychrophila |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
monomer | Desulfotalea psychrophila |
170000 | - |
determined by Blue-Native PAGE, homotetramer | Desulfotalea psychrophila |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP | Desulfotalea psychrophila | - |
diphosphate + cyclic di-3',5'-guanylate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Desulfotalea psychrophila | Q6ARU5 | - |
- |
Purification (Comment) | Organism |
---|---|
using a TALON metal affinity resin and a Superdex 200 column | Desulfotalea psychrophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP | - |
Desulfotalea psychrophila | diphosphate + cyclic di-3',5'-guanylate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 42000 | Desulfotalea psychrophila |
Synonyms | Comment | Organism |
---|---|---|
diguanylate cyclase | - |
Desulfotalea psychrophila |
HemDGC | - |
Desulfotalea psychrophila |
heme-containing diguanylate cyclase | - |
Desulfotalea psychrophila |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
activity assay | Desulfotalea psychrophila |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
activity assay | Desulfotalea psychrophila |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Desulfotalea psychrophila |