Literature summary for 2.7.7.65 extracted from

Neunuebel, M.R.; Golden, J.W.
The Anabaena sp. strain PCC 7120 gene all2874 encodes a diguanylate cyclase and is required for normal heterocyst development under high-light growth conditions (2008), J. Bacteriol., 190, 6829-6836.

Search for more literature for 2.7.7.65

Cloned(Commentary)

Cloned (Comment)	Organism
630-bp internal fragment from bacterial DNA in suicide plasmid pRL277 for construction of suicide plasmid pAM4108 and generation of mutant strains by homologous recombination, complete ORF in pAM2770 for generation of pAM4114 for complementation studies, complete ORF in pEZ-30b(+) (pAM4097) for expression with C-terminal hexa-His-tag in Escherichia coli BL21(DE3)	Anabaena sp.

Organism

Organism	UniProt	Comment	Textmining
Anabaena sp.	-	strain PCC 7120	-

Purification (Commentary)

Purification (Comment)	Organism
from bacterial lysate by metal affinity chromatography using HisTrap HP column and FPLC (elution: linear imidazole gradient) and dialysis	Anabaena sp.

Source Tissue

Source Tissue	Comment	Organism	Textmining
heterocyst	heterocyst differentiation pathway (upstream of HetR), decreased heterocyst frequency and block of heterocyst differentiation within 4 days in mutant strain depleted for all2874 gene and grown in absence of source of combined nitrogen	Anabaena sp.	-
vegetative cell	-	Anabaena sp.	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GTP + GTP	pH 8, 60 min, 25°C, 10 mM MgCl2	Anabaena sp.	cyclic di-3',5'-guanylate + diphosphate + diphosphate	reaction stop by 0.083 mM EDTA	?

Synonyms

Synonyms	Comment	Organism
ALL2874	-	Anabaena sp.
ALL2874	encoded by gene all2874	Anabaena sp.
diguanylate cyclase	-	Anabaena sp.

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Release 2023.1 (January 2023)