Literature summary for 2.7.7.60 extracted from

Richard, S.B.; Bowman, M.E.; Kwiatkowski, W.; Kang, L.; Chow, C.; Lillo, A.M.; Cane, D.E.; Noel, J.P.
Structure of 4-diphosphocytidyl-2-C-methylerythritol synthetase involved in mevalonate-independent isoprenoid biosynthesis (2001), Nature Struct. Biol., 8, 641-648.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3)	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
vapor diffusion method at 4°C in hanging drops, crystals belong to space group C2	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
K213S	effective turnover rate is ca. 0.4% of the rate of wild type, retains capacity to form product with significantly reduced efficiency	Escherichia coli
K27A	amino acid plays an essential role in catalysis because the effective turnover rate is ca. 0.025% of the rate of wild type enzyme	Escherichia coli
K27S	amino acid plays an essential role in catalysis because the effective turnover rate is ca. 0.025% of the rate of wild type enzyme	Escherichia coli

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	essential for activity	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
CTP + 2-C-methyl-D-erythritol-4-phosphate	Mycobacterium tuberculosis	-	diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol	-	?
CTP + 2-C-methyl-D-erythritol-4-phosphate	Plasmodium falciparum	-	diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol	-	?
CTP + 2-C-methyl-D-erythritol-4-phosphate	Escherichia coli	third step of biosynthesis	diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol	-	?
CTP + 2-C-methyl-D-erythritol-4-phosphate	Escherichia coli	part of mevalonate-indpendent pathway for isoprenoid biosynthesis	diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-
Mycobacterium tuberculosis	-	-	-
Plasmodium falciparum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni2+-nitrilotriacetic acid column	Escherichia coli

Reaction

Reaction	Comment	Organism	Reaction ID
CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol	cytidyltransferase family. Active substrate site consists of a glycine-rich loop spanning Pro13-Arg20. Three-dimensional structure of enzyme is figured out	Escherichia coli	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
CTP + 2-C-methyl-D-erythritol 4-phosphate	-	Escherichia coli	diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol	-	?
CTP + 2-C-methyl-D-erythritol-4-phosphate	-	Mycobacterium tuberculosis	diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol	-	?
CTP + 2-C-methyl-D-erythritol-4-phosphate	-	Plasmodium falciparum	diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol	-	?
CTP + 2-C-methyl-D-erythritol-4-phosphate	third step of biosynthesis	Escherichia coli	diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol	-	?
CTP + 2-C-methyl-D-erythritol-4-phosphate	part of mevalonate-indpendent pathway for isoprenoid biosynthesis	Escherichia coli	diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol	-	?

Subunits

Subunits	Comment	Organism
dimer	homodimer	Escherichia coli
dimer	each subunit contains a globular core domain with an alpha/beta sturcture and and one smaller subdomain	Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Escherichia coli

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Escherichia coli

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Release 2023.1 (January 2023)