Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.59 extracted from

  • Garcia, E.; Rhee, S.G.
    Cascade control of Escherichia coli glutamine synthetase (1983), J. Biol. Chem., 258, 2246-2253.
    View publication on PubMed
Search for more literature for 2.7.7.59

Cloned(Commentary)

Cloned (Comment) Organism
gene glnD, overexpression from plasmid in an Escherichia coli strain Escherichia coli

General Stability

General Stability Organism
freezing and thawing of the purified enzyme results in 35-50% loss of activity Escherichia coli
purification drastically reduces enzyme stability Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
CMP inhibits uridylyl removing activity Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
94000
-
 monomeric enzyme form, HPLC gel filtration Escherichia coli
186000
-
 dimeric enzyme form, HPLC gel filtration Escherichia coli
550000
-
 hexameric form, HPLC gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UTP + [protein-PII] Escherichia coli the enzyme is regulated by Gln and controls the activity of PII signal transduction protein diphosphate + uridylyl-[protein-PII]
-
 r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 gene glnD
-

Purification (Commentary)

Purification (Comment) Organism
1720fold to homogeneity from recombinant overexpressing strain Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Escherichia coli

Storage Stability

Storage Stability Organism
-70°C, several months without loss of activity Escherichia coli
4°C, purified enzyme, in presence of ATP and 0.2 M KCl, 10 h, loss of 15-20% activity compared to storage conditions at -5°C Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UTP + [protein-PII]
-
 Escherichia coli diphosphate + uridylyl-[protein-PII]
-
 r
UTP + [protein-PII] the enzyme is regulated by Gln and controls the activity of PII signal transduction protein Escherichia coli diphosphate + uridylyl-[protein-PII]
-
 r

Subunits

Subunits Comment Organism
dimer 2 * 94000, the enzyme is detected as monomeric, dimeric and hexameric form, HPLC after equilibration with SDS Escherichia coli
hexamer 6 * 94000, the enzyme is detected as monomeric, dimeric and hexameric form, HPLC after equilibration with SDS Escherichia coli
monomer 1 * 94000, the enzyme is detected as monomeric, dimeric and hexameric form, HPLC after equilibration with SDS Escherichia coli