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Literature summary for 2.7.7.52 extracted from

  • Yamashita, S.; Nagaike, T.; Tomita, K.
    Crystal structure of the Lin28-interacting module of human terminal uridylyltransferase that regulates let-7 expression (2019), Nat. Commun., 10, 1960 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.52

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
Lin28-interacting module of TUT4, sitting drop vapor diffusion method, using 100 mM HEPES-NaOH, pH 7.0, 20% (w/v) PEG3350, 3% (v/v) 2-methyl-2,4-pentanediol, 200mM ammonium citrate, and 3% (w/v) 1,5 diaminopentane dihydrochloride Homo sapiens

Protein Variants

Protein Variants Comment Organism
C306A/C309A the mutant does not oligo-uridylylate pre-let-7a-1 RNA in the presence of Lin28A Homo sapiens
H405A/K452A the mutant shows reduced oligo-uridylylation of pre-let-7a-1 RNA in the presence of Lin28A compared to the wild type enzyme Homo sapiens
K321A/K324A the mutant shows severely reduced oligo-uridylylation of pre-let-7a-1 RNA in the presence of Lin28A compared to the wild type enzyme Homo sapiens
K326A/R327A the mutant shows severely reduced oligo-uridylylation of pre-let-7a-1 RNA in the presence of Lin28A compared to the wild type enzyme Homo sapiens
K329A/K330A the mutant shows strongly reduced oligo-uridylylation of pre-let-7a-1 RNA in the presence of Lin28A compared to the wild type enzyme Homo sapiens
R669A/R670A the mutant shows strongly reduced oligo-uridylylation of pre-let-7a-1 RNA in the presence of Lin28A compared to the wild type enzyme Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ contains zinc Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UTP + precursor let-7 RNAn Homo sapiens the interaction between the Lin28:pre-let-7 complex and the N-terminal Lin28-interacting module of TUT4 is required for prelet-7 oligo-uridylylation by the C-terminal catalytic module of TUT4/7 diphosphate + precursor let-7 RNAn+1
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q5TAX3
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography, HiTrap heparin column chromatography and Superdex 200 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UTP + precursor let-7 RNAn the interaction between the Lin28:pre-let-7 complex and the N-terminal Lin28-interacting module of TUT4 is required for prelet-7 oligo-uridylylation by the C-terminal catalytic module of TUT4/7 Homo sapiens diphosphate + precursor let-7 RNAn+1
-
 ?
UTP + precursor let-7a-1 RNAn
-
 Homo sapiens diphosphate + precursor let-7a-1 RNAn+1
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 ?

Synonyms

Synonyms Comment Organism
terminal uridylyltransferase
-
 Homo sapiens
TUT4 isoform Homo sapiens