Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
Lin28-interacting module of TUT4, sitting drop vapor diffusion method, using 100 mM HEPES-NaOH, pH 7.0, 20% (w/v) PEG3350, 3% (v/v) 2-methyl-2,4-pentanediol, 200mM ammonium citrate, and 3% (w/v) 1,5 diaminopentane dihydrochloride | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C306A/C309A | the mutant does not oligo-uridylylate pre-let-7a-1 RNA in the presence of Lin28A | Homo sapiens |
H405A/K452A | the mutant shows reduced oligo-uridylylation of pre-let-7a-1 RNA in the presence of Lin28A compared to the wild type enzyme | Homo sapiens |
K321A/K324A | the mutant shows severely reduced oligo-uridylylation of pre-let-7a-1 RNA in the presence of Lin28A compared to the wild type enzyme | Homo sapiens |
K326A/R327A | the mutant shows severely reduced oligo-uridylylation of pre-let-7a-1 RNA in the presence of Lin28A compared to the wild type enzyme | Homo sapiens |
K329A/K330A | the mutant shows strongly reduced oligo-uridylylation of pre-let-7a-1 RNA in the presence of Lin28A compared to the wild type enzyme | Homo sapiens |
R669A/R670A | the mutant shows strongly reduced oligo-uridylylation of pre-let-7a-1 RNA in the presence of Lin28A compared to the wild type enzyme | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | contains zinc | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UTP + precursor let-7 RNAn | Homo sapiens | the interaction between the Lin28:pre-let-7 complex and the N-terminal Lin28-interacting module of TUT4 is required for prelet-7 oligo-uridylylation by the C-terminal catalytic module of TUT4/7 | diphosphate + precursor let-7 RNAn+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q5TAX3 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography, HiTrap heparin column chromatography and Superdex 200 gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UTP + precursor let-7 RNAn | the interaction between the Lin28:pre-let-7 complex and the N-terminal Lin28-interacting module of TUT4 is required for prelet-7 oligo-uridylylation by the C-terminal catalytic module of TUT4/7 | Homo sapiens | diphosphate + precursor let-7 RNAn+1 | - |
? | |
UTP + precursor let-7a-1 RNAn | - |
Homo sapiens | diphosphate + precursor let-7a-1 RNAn+1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
terminal uridylyltransferase | - |
Homo sapiens |
TUT4 | isoform | Homo sapiens |