Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli One Shot BL21 Star (DE3) cells | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
enzyme in complex with either NAD or NMN, hanging drop vapor diffusion method, using either 0.2 M sodium nitrate, 20% PEG-3350 or 1 M LiCl2, 0.1 M HEPES, pH 7.0, and 10% PEG-6000 | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
E174Q | inactive | Escherichia coli |
E178Q | inactive | Escherichia coli |
E219Q | inactive | Escherichia coli |
F160A | inactive | Escherichia coli |
P236A | the mutant shows 10% of wild type activity | Escherichia coli |
R69A | the mutant shows high activity as compared to the wild type enzyme | Escherichia coli |
Y124A | inactive | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 10 mM used in assay conditions | Escherichia coli | |
Zn2+ | contains zinc | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD-capped RNA + H2O | Escherichia coli | NudC is a single-strand-specific RNA-decapping enzyme with a strong preference for a purine as first nucleotide | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography and Superdex G200 gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-NAD-capped RNA + ? | NudC is a single-strand-specific RNA-decapping enzyme with a strong preference for a purine as first nucleotide. NudC prefers NAD-RNA over NAD(H) by several orders of magnitude. The enzyme has strong preference for RNAs with at least three unpaired nucleotides at their 5' ends | Escherichia coli | ? | - |
? | |
additional information | an RNAI mutant with a paired 5' end, as well as native Escherichia coli 5S rRNA (also possessing a paired 5' end) are resistant to enzyme processing | Escherichia coli | ? | - |
- |
|
NAD(H) + H2O | c.f. 3.6.1.22. The enzyme is not very efficient in hydrolyzing NAD | Escherichia coli | AMP + NMN(H) | - |
? | |
NAD-capped RNA + H2O | NudC is a single-strand-specific RNA-decapping enzyme with a strong preference for a purine as first nucleotide | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
NAD-decapping enzyme | - |
Escherichia coli |
NudC | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Escherichia coli |