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Literature summary for 2.7.7.50 extracted from

  • Takagi, Y.; Sindkar, S.; Ekonomidis, D.; Hall, M.P.; Ho, C.K.
    Trypanosoma brucei encodes a bifunctional capping enzyme essential for cap 4 formation on the spliced leader RNA (2007), J. Biol. Chem., 282, 15995-16005.
    View publication on PubMed
Search for more literature for 2.7.7.50

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(ROS2) cells Trypanosoma brucei

Inhibitors

Inhibitors Comment Organism Structure
diphosphate 95% inhibition at 0.5 mM Trypanosoma brucei

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Trypanosoma brucei
Mn2+ Mn2+ is a more effective cofactor than Mg2+ at concentrations below 0.5 mM but is progressively less at higher concentrations Trypanosoma brucei
additional information Ca2+, Cu2+, and Zn2+ do not support the activity at 2 mM Trypanosoma brucei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 SDS-PAGE Trypanosoma brucei

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel-agarose chromatography Trypanosoma brucei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + pp(5')RNA
-
 Trypanosoma brucei G(5')ppp(5')RNA + diphosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 42000, SDS-PAGE Trypanosoma brucei

Synonyms

Synonyms Comment Organism
cap guanylyltransferase-methyltransferase bifunctional capping enzyme consisting of an amino-terminal guanylyltransferase domain and a carboxyl-terminal methyltransferase domain Trypanosoma brucei
TbCgm1
-
 Trypanosoma brucei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7.5 in 50 mM Tris-HCl Trypanosoma brucei

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 7 the amount of G(5')ppp(5')RNA formed at pH 5.5 is 50% of the amount formed at pH 7 Trypanosoma brucei