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Literature summary for 2.7.7.50 extracted from

  • Guarino, L.A.; Jin, J.; Dong, W.
    Guanylyltransferase activity of the LEF-4 subunit of baculovirus RNA polymerase (1998), J. Virol., 72, 10003-10010.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.50

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of LEF-4 subunit in Spodoptera frugiperda cells by recombinant virus infection under control of polyhedrin promotor Autographa californica nucleopolyhedrovirus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Autographa californica nucleopolyhedrovirus

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information single recombinant LEF-4 subunit is cytosolic, but the whole RNA polymerase complex is targeted to the nucleus in baculovirus infected cells Autographa californica nucleopolyhedrovirus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ maximal at 20-40 mM, LEF-4 subunit Autographa californica nucleopolyhedrovirus
Mg2+ enzyme-GMP intermediate formation is strictly dependent on divalent cations, satisfied by Mn2+ or Mg2+, Mn2+ is slightly preferred Autographa californica nucleopolyhedrovirus
Mn2+ enzyme-GMP intermediate formation is strictly dependent on divalent cations, satisfied by Mn2+ or Mg2+, Mn2+ is slightly preferred Autographa californica nucleopolyhedrovirus
Mn2+ maximal at 5 mM, LEF-4 subunit Autographa californica nucleopolyhedrovirus
additional information requirement for divalent cations Autographa californica nucleopolyhedrovirus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
 LEF-4 subunit at 0.4 M KCl, gel filtration Autographa californica nucleopolyhedrovirus
114200
-
 LEF-4 subunit forming dimers in solution by weak ionic interactions, gel filtration Autographa californica nucleopolyhedrovirus

Organism

Organism UniProt Comment Textmining
Autographa californica nucleopolyhedrovirus
-
 LEF-4 subunit of DNA-dependent RNA-polymerase
-
Autographa californica nucleopolyhedrovirus
-
 i.e. baculovirus
-

Purification (Commentary)

Purification (Comment) Organism
recombinant LEF-4 subunit from overexpressing Spodoptera frugiperda cells Autographa californica nucleopolyhedrovirus

Reaction

Reaction Comment Organism Reaction ID
GTP + (5')ppPur-mRNA = diphosphate + G(5')pppPur-mRNA conserved KXDG-motif Autographa californica nucleopolyhedrovirus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dGTP + pp(5')RNA low activity Autographa californica nucleopolyhedrovirus dG(5')ppp(5')RNA + diphosphate deoxyguanosine residue linked 5' through three phosphates to the 5' position of the terminal residue r
GTP + pp(5')RNA specific for GTP Autographa californica nucleopolyhedrovirus G(5')ppp(5')RNA + diphosphate guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue r
GTP + pp(5')RNA GMP is linked to the enzyme via a phosphoamide bond Autographa californica nucleopolyhedrovirus G(5')ppp(5')RNA + diphosphate guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue r
GTP + pp(5')RNA formation of a covalent intermediate enzyme-GMP Autographa californica nucleopolyhedrovirus G(5')ppp(5')RNA + diphosphate guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue r
GTPgammaS + pp(5')RNA no activity Autographa californica nucleopolyhedrovirus ?
-
 ?
additional information no activity with ATP, CTP, UTP Autographa californica nucleopolyhedrovirus ?
-
 ?

Subunits

Subunits Comment Organism
? 1 * 54000, LEF-4 subunit, SDS-PAGE, monomeric in solution as recombinant protein, in vivo part of RNA polymerase Autographa californica nucleopolyhedrovirus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Autographa californica nucleopolyhedrovirus