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Literature summary for 2.7.7.50 extracted from

  • Wang, D.; Shatkin, A.J.
    Synthesis of Gp4N and Gp3N compounds by guanylyltransferase purified from yeast (1984), Nucleic Acids Res., 12, 2303-2315.
    View publication on PubMedView publication on EuropePMC

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
231fold Saccharomyces cerevisiae
contains little or no RNA 5'-triphosphatase or methyltransferase activity Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00003
-
purified enzyme Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + pp(5')ApGp
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Saccharomyces cerevisiae G(5')ppp(5')ApGp + diphosphate guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue ?
GTP + pp(5')RNA formation of a covalent intermediate enzyme-GMP Saccharomyces cerevisiae G(5')ppp(5')RNA + diphosphate guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue ir
GTP + ppp(5')RNA
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Saccharomyces cerevisiae G(5')pppp(5')RNA + diphosphate guanosine residue linked 5' through four phosphates to the 5' position of the terminal residue ?
additional information specificity overview Saccharomyces cerevisiae ?
-
?

Subunits

Subunits Comment Organism
? x * 45000-52000, SDS-PAGE Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Saccharomyces cerevisiae