Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | can partially replace Mg2+ in activation, inhibits in presence of Mg2+ | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0005 | - |
pp(5')GCC(A2,U2G)n | 2 Km values: 0.0005 and 0.004 mM, pH 7.0, 37°C | Saccharomyces cerevisiae | |
0.004 | - |
pp(5')GCC(A2,U2G)n | 2 Km values: 0.0005 and 0.004 mM, pH 7.0, 37°C | Saccharomyces cerevisiae | |
2 | - |
diphosphate | pH 7.0, 37°C | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Saccharomyces cerevisiae | |
Mg2+ | maximal activity at 3 mM | Saccharomyces cerevisiae | |
Mn2+ | can partially replace Mg2+ in activation | Saccharomyces cerevisiae | |
Mn2+ | 10% of the activity with Mg2+ | Saccharomyces cerevisiae | |
Mn2+ | maximal activity at 1 mM | Saccharomyces cerevisiae | |
Mn2+ | inhibits in presence of Mg2+ | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
physically associated with mRNA 5'-triphosphatase activity | Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-80°C, reaction intermediate enzyme-GMP, purified, stable for at least several weeks | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + pp(5')GCC(A2,U2G)n | - |
Saccharomyces cerevisiae | ? | - |
? | |
GTP + pp(5')RNA | reversible formation of a covalent intermediate enzyme-GMP | Saccharomyces cerevisiae | G(5')ppp(5')RNA + diphosphate | guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue | ? | |
GTP + pp(5')RNA | formation of a covalent intermediate enzyme-GMP | Saccharomyces cerevisiae | G(5')ppp(5')RNA + diphosphate | guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue | ? | |
GTP + ppGpC | - |
Saccharomyces cerevisiae | G(5')ppp(5')GpC + diphosphate | - |
ir | |
GTP + ppp(5')RNA | - |
Saccharomyces cerevisiae | G(5')pppp(5')RNA + diphosphate | - |
? | |
additional information | no acceptor: ppGp | Saccharomyces cerevisiae | ? | - |
? | |
additional information | in absence of acceptor RNA, the enzyme forms a nucleotidyl intermediate by phosphoamidate linkage of GMP | Saccharomyces cerevisiae | ? | - |
? | |
additional information | enzyme also catalyzes GTP-diphosphate exchange | Saccharomyces cerevisiae | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.4 | 7.9 | pH 6.4: about 30% of activity maximum, pH 7.9: about 25% of activity maximum | Saccharomyces cerevisiae |