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Literature summary for 2.7.7.50 extracted from

  • Itoh, N.; Mizumoto, K.; Kaziro, Y.
    Messenger RNA guanlyltransferase from Saccharomyces cerevisiae. II. Catalytic properties (1984), J. Biol. Chem., 259, 13930-13936.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Mn2+ can partially replace Mg2+ in activation, inhibits in presence of Mg2+ Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0005
-
pp(5')GCC(A2,U2G)n 2 Km values: 0.0005 and 0.004 mM, pH 7.0, 37°C Saccharomyces cerevisiae
0.004
-
pp(5')GCC(A2,U2G)n 2 Km values: 0.0005 and 0.004 mM, pH 7.0, 37°C Saccharomyces cerevisiae
2
-
diphosphate pH 7.0, 37°C Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Saccharomyces cerevisiae
Mg2+ maximal activity at 3 mM Saccharomyces cerevisiae
Mn2+ can partially replace Mg2+ in activation Saccharomyces cerevisiae
Mn2+ 10% of the activity with Mg2+ Saccharomyces cerevisiae
Mn2+ maximal activity at 1 mM Saccharomyces cerevisiae
Mn2+ inhibits in presence of Mg2+ Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
physically associated with mRNA 5'-triphosphatase activity Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
-80°C, reaction intermediate enzyme-GMP, purified, stable for at least several weeks Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + pp(5')GCC(A2,U2G)n
-
Saccharomyces cerevisiae ?
-
?
GTP + pp(5')RNA reversible formation of a covalent intermediate enzyme-GMP Saccharomyces cerevisiae G(5')ppp(5')RNA + diphosphate guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue ?
GTP + pp(5')RNA formation of a covalent intermediate enzyme-GMP Saccharomyces cerevisiae G(5')ppp(5')RNA + diphosphate guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue ?
GTP + ppGpC
-
Saccharomyces cerevisiae G(5')ppp(5')GpC + diphosphate
-
ir
GTP + ppp(5')RNA
-
Saccharomyces cerevisiae G(5')pppp(5')RNA + diphosphate
-
?
additional information no acceptor: ppGp Saccharomyces cerevisiae ?
-
?
additional information in absence of acceptor RNA, the enzyme forms a nucleotidyl intermediate by phosphoamidate linkage of GMP Saccharomyces cerevisiae ?
-
?
additional information enzyme also catalyzes GTP-diphosphate exchange Saccharomyces cerevisiae ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
6.4 7.9 pH 6.4: about 30% of activity maximum, pH 7.9: about 25% of activity maximum Saccharomyces cerevisiae