Any feedback?
Literature summary for 2.7.7.50 extracted from
- Catalytic activity of vaccinia mRNA capping enzyme subunits coexpressed in Escherichia coli (1990), J. Biol. Chem., 265, 11960-11966.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of viral D1 and D12 orfs encoded subunits in Escherichia coli BL21(DE3) | Vaccinia virus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31000 | - |
x * 95000 + x * 31000, SDS-PAGE | Vaccinia virus |
| 95000 | - |
x * 95000 + x * 31000, SDS-PAGE | Vaccinia virus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vaccinia virus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 1000fold, recombinant from Escherichia coli | Vaccinia virus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.58 | - |
purified recombinant enzyme | Vaccinia virus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + pp(5')RNA | - |
Vaccinia virus | G(5')ppp(5')RNA + diphosphate | guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue | ? |  |
| additional information | activities associated with vaccinia capping enzyme complex: 1. GTP-RNA guanylyltransferase, 2. RNA (guanine-7)-methyltransferase, 3. RNA triphosphatase, 4. GTP-diphosphate exchange, 5. nucleoside triphosphate phosphorylase | Vaccinia virus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 95000 + x * 31000, SDS-PAGE | Vaccinia virus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | activities associated with vaccinia capping enzyme complex: 1. GTP-RNA guanylyltransferase, 2. RNA (guanine-7)-methyltransferase, 3. RNA triphosphatase, 4. GTP-diphosphate exchange, 5. nucleoside triphosphate phosphorylase | Vaccinia virus |
| mRNA capping enzyme | activities associated with vaccinia capping enzyme complex: 1. GTP-RNA guanylyltransferase, 2. RNA (guanine-7)-methyltransferase, 3. RNA triphosphatase, 4. GTP-PPi exchange, 5. nucleoside triphosphate phosphorylase | Vaccinia virus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Vaccinia virus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Vaccinia virus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
