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Literature summary for 2.7.7.50 extracted from
- Capping enzyme (1982), The Enzymes, 3rd. Ed. (Boyer, P. D. , ed. ), 15, 245-265.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| S-adenosylmethionine | stimulates up to 2fold | Vaccinia virus |  |
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | valuable in a variety of analytical studies of RNA | Vaccinia virus |
| biotechnology | enzyme can be used as a tool for specific 5'-end-labeling of mRNA | Vaccinia virus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diphosphate | - |
Homo sapiens | |
| diphosphate | strong product inhibition | Vaccinia virus | |
| additional information | no inhibition by S-adenosylmethionine and S-adenosylhomocysteine | Homo sapiens | |
| additional information | - |
Vaccinia virus | |
| N-ethylmaleimide | - |
Homo sapiens | |
| NaCl | 90% inhibition at 0.3 M | Vaccinia virus | |
| phosphate | above 20 mM | Vaccinia virus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000285 | - |
pp(5')ApGp | pH 7.8, 37°C | Homo sapiens | |
| 0.015 | - |
GTP | pH 7.8, 37°C | Vaccinia virus | |
| 0.019 | - |
diphosphate terminated poly(A) with an average chain length of 2000 nucleotides | - |
Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| nucleus | - |
Homo sapiens | 5634 | - |
| nucleus | - |
Rattus norvegicus | 5634 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Homo sapiens | |
| Mg2+ | required | Vaccinia virus | |
| Mg2+ | can partially replace Mn2+ in activation | Homo sapiens | |
| Mg2+ | can partially replace Mn2+ in activation | Rattus norvegicus | |
| Mg2+ | 25% of the activity with Mn2+ | Homo sapiens | |
| Mn2+ | required | Homo sapiens | |
| Mn2+ | required | Rattus norvegicus | |
| Mn2+ | can partially replace Mg2+ in activation | Homo sapiens | |
| Mn2+ | can partially replace Mg2+ in activation | Rattus norvegicus | |
| Mn2+ | can partially replace Mg2+ in activation | Vaccinia virus | |
| Mn2+ | maximal activity at 2 mM | Homo sapiens | |
| additional information | no activation by Ca2+ or Zn2+ | Vaccinia virus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26400 | - |
x * 95000, subunit containing the active site + x * 26400, transguanylyltransferase subunit, SDS-PAGE | Vaccinia virus |
| 48500 | - |
sucrose density gradient sedimentation | Homo sapiens |
| 65000 | - |
gel filtration | Rattus norvegicus |
| 95000 | - |
x * 95000, subunit containing the active site + x * 26400, transguanylyltransferase subunit, SDS-PAGE | Vaccinia virus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + pp(5')RNA | Homo sapiens | - |
G(5')ppp(5')RNA + diphosphate | guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue | ir | |
| GTP + pp(5')RNA | Rattus norvegicus | - |
G(5')ppp(5')RNA + diphosphate | guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue | r | |
| GTP + pp(5')RNA | Vaccinia virus | - |
G(5')ppp(5')RNA + diphosphate | guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue | r | |
| GTP + ppp(5')RNA | Homo sapiens | - |
G(5')pppp(5')RNA + diphosphate | guanosine residue linked 5' through four phosphates to the 5' position of the terminal residue | ir | |
| GTP + ppp(5')RNA | Rattus norvegicus | - |
G(5')pppp(5')RNA + diphosphate | guanosine residue linked 5' through four phosphates to the 5' position of the terminal residue | r | |
| GTP + ppp(5')RNA | Vaccinia virus | - |
G(5')pppp(5')RNA + diphosphate | guanosine residue linked 5' through four phosphates to the 5' position of the terminal residue | r | |
| additional information | Homo sapiens | mRNA capping enzyme | ? | - |
? | |
| additional information | Rattus norvegicus | mRNA capping enzyme | ? | - |
? | |
| additional information | Vaccinia virus | mRNA capping enzyme | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
HeLa cells | - |
| Rattus norvegicus | - |
- |
- |
| Vaccinia virus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Vaccinia virus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| GTP + (5')ppPur-mRNA = diphosphate + G(5')pppPur-mRNA | mechanism of capping | Homo sapiens | |
| GTP + (5')ppPur-mRNA = diphosphate + G(5')pppPur-mRNA | mechanism of capping | Rattus norvegicus | |
| GTP + (5')ppPur-mRNA = diphosphate + G(5')pppPur-mRNA | mechanism of capping | Vaccinia virus | |
| GTP + (5')ppPur-mRNA = diphosphate + G(5')pppPur-mRNA | mRNA containing a guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue | Homo sapiens | |
| GTP + (5')ppPur-mRNA = diphosphate + G(5')pppPur-mRNA | mRNA containing a guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue | Rattus norvegicus | |
| GTP + (5')ppPur-mRNA = diphosphate + G(5')pppPur-mRNA | mRNA containing a guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue | Vaccinia virus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HeLa cell | - |
Homo sapiens | - |
| liver | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dGTP + pp(5')RNA | - |
Vaccinia virus | dG(5')ppp(5')RNA + diphosphate | deoxyguanosine residue linked 5' through three phosphates to the 5' position of the terminal residue | r | |
| dGTP + pp(5')RNA | no activity | Homo sapiens | dG(5')ppp(5')RNA + diphosphate | - |
? | |
| GTP + diphosphate terminated poly(A) with an average chain length of 2000 nucleotides | - |
Homo sapiens | ? | - |
? | |
| GTP + pp(5')ApGp | - |
Homo sapiens | G(5')ppp(5')ApGp + diphosphate | - |
ir | |
| GTP + pp(5')GpCpC | - |
Rattus norvegicus | G(5')ppp(5')GpCpC + diphosphate | guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue | r | |
| GTP + pp(5')RNA | - |
Homo sapiens | G(5')ppp(5')RNA + diphosphate | guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue | ir | |
| GTP + pp(5')RNA | - |
Rattus norvegicus | G(5')ppp(5')RNA + diphosphate | guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue | r | |
| GTP + pp(5')RNA | - |
Vaccinia virus | G(5')ppp(5')RNA + diphosphate | guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue | r | |
| GTP + pp(5')RNA | specific for GTP | Vaccinia virus | G(5')ppp(5')RNA + diphosphate | guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue | r | |
| GTP + pp(5')RNA | no apparent base specificity for the penultimate nucleotide, a variety of synthetic homoribopolymers and naturally occuring mRNAs are effective substrates, vaccinia virus | Vaccinia virus | G(5')ppp(5')RNA + diphosphate | guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue | r | |
| GTP + pp(5')RNA | the acceptor is diphosphate terminated poly(A) | Homo sapiens | G(5')ppp(5')RNA + diphosphate | guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue | ir | |
| GTP + pp(5')RNA | the acceptor is diphosphate terminated poly(A) | Homo sapiens | G(5')ppp(5')RNA + diphosphate | guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue | r | |
| GTP + pp(5')RNA | the acceptor is diphosphate terminated poly(A) | Vaccinia virus | G(5')ppp(5')RNA + diphosphate | guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue | r | |
| GTP + ppApG | - |
Vaccinia virus | G(5')ppp(5')ApG + diphosphate | guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue | r | |
| GTP + ppGpC | - |
Homo sapiens | G(5')ppp(5')GpC + diphosphate | - |
ir | |
| GTP + ppGpC | - |
Vaccinia virus | G(5')ppp(5')GpC + diphosphate | guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue | ir | |
| GTP + ppp(5')ApG | - |
Homo sapiens | G(5')pppp(5')ApG + diphosphate | - |
ir | |
| GTP + ppp(5')ApG | - |
Vaccinia virus | G(5')pppp(5')ApG + diphosphate | guanosine residue linked 5' through four phosphates to the 5' position of the terminal residue | r | |
| GTP + ppp(5')RNA | - |
Homo sapiens | G(5')pppp(5')RNA + diphosphate | guanosine residue linked 5' through four phosphates to the 5' position of the terminal residue | ir | |
| GTP + ppp(5')RNA | - |
Rattus norvegicus | G(5')pppp(5')RNA + diphosphate | guanosine residue linked 5' through four phosphates to the 5' position of the terminal residue | r | |
| GTP + ppp(5')RNA | - |
Vaccinia virus | G(5')pppp(5')RNA + diphosphate | guanosine residue linked 5' through four phosphates to the 5' position of the terminal residue | r | |
| GTP + pppGpCpC | - |
Rattus norvegicus | G(5')pppp(5')GpCpC + diphosphate | guanosine residue linked 5' through four phosphates to the 5' position of the terminal residue | r | |
| GTPgammaS + pp(5')RNA | - |
Vaccinia virus | ? | - |
r | |
| ITP + pp(5')RNA | - |
Homo sapiens | I(5')ppp(5')RNA + diphosphate | inosine residue linked 5' through three phosphates to the 5' position of the terminal residue | ir | |
| additional information | activities associated with vaccinia capping enzyme complex: 1. GTP-RNA guanylyltransferase, 2. RNA (guanine-7)-methyltransferase, 3. RNA triphosphatase, 4. GTP-diphosphate exchange, 5. nucleoside triphosphate phosphorylase | Vaccinia virus | ? | - |
? | |
| additional information | no activity with GMP | Vaccinia virus | ? | - |
? | |
| additional information | no acceptor: RNA with 5'-hydroxyl terminus | Vaccinia virus | ? | - |
? | |
| additional information | no activity with GDP | Homo sapiens | ? | - |
? | |
| additional information | no activity with GDP | Vaccinia virus | ? | - |
? | |
| additional information | no donor: 7-methylGTP | Homo sapiens | ? | - |
? | |
| additional information | no donor: 7-methylGTP | Vaccinia virus | ? | - |
? | |
| additional information | no acceptor: RNA with a single 5'-terminal phosphate | Vaccinia virus | ? | - |
? | |
| additional information | enzyme also catalyzes GTP-diphosphate exchange | Homo sapiens | ? | - |
? | |
| additional information | enzyme also catalyzes GTP-diphosphate exchange | Rattus norvegicus | ? | - |
? | |
| additional information | enzyme also catalyzes GTP-diphosphate exchange | Vaccinia virus | ? | - |
? | |
| additional information | no activity with ATP, CTP, UTP | Homo sapiens | ? | - |
? | |
| additional information | no activity with ATP, CTP, UTP | Vaccinia virus | ? | - |
? | |
| additional information | mRNA capping enzyme | Homo sapiens | ? | - |
? | |
| additional information | mRNA capping enzyme | Rattus norvegicus | ? | - |
? | |
| additional information | mRNA capping enzyme | Vaccinia virus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 95000, subunit containing the active site + x * 26400, transguanylyltransferase subunit, SDS-PAGE | Vaccinia virus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | activities associated with vaccinia capping enzyme complex: 1. GTP-RNA guanylyltransferase, 2. RNA (guanine-7)-methyltransferase, 3. RNA triphosphatase, 4. GTP-diphosphate exchange, 5. nucleoside triphosphate phosphorylase | Vaccinia virus |
| mRNA capping enzyme | activities associated with vaccinia capping enzyme complex: 1. GTP-RNA guanylyltransferase, 2. RNA (guanine-7)-methyltransferase, 3. RNA triphosphatase, 4. GTP-PPi exchange, 5. nucleoside triphosphate phosphorylase | Vaccinia virus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
| 37 | - |
assay at | Rattus norvegicus |
| 37 | - |
assay at | Vaccinia virus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
5 min, complete inactivation | Vaccinia virus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Homo sapiens |
| 7.8 | - |
Tris-HCl buffer | Vaccinia virus |
| 7.8 | - |
assay at | Homo sapiens |
| 7.8 | - |
assay at | Rattus norvegicus |
| 7.8 | - |
assay at | Vaccinia virus |
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