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Literature summary for 2.7.7.48 extracted from

  • Sarin, L.P.; Poranen, M.M.; Lehti, N.M.; Ravantti, J.J.; Koivunen, M.R.; Aalto, A.P.; van Dijk, A.A.; Stuart, D.I.; Grimes, J.M.; Bamford, D.H.
    Insights into the pre-initiation events of bacteriophage phi 6 RNA-dependent RNA polymerase: towards the assembly of a productive binary complex (2009), Nucleic Acids Res., 37, 1182-1192.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and muztant enzymes in Escherichia coli strain BL21(DE3) Cystovirus phi6

Protein Variants

Protein Variants Comment Organism
E139Q site-directed mutagenesis in the plough region Cystovirus phi6
E165A site-directed mutagenesis in the plough region Cystovirus phi6
E165Q site-directed mutagenesis in the plough region Cystovirus phi6
E168Q site-directed mutagenesis in the plough region Cystovirus phi6
E172Q site-directed mutagenesis in the plough region Cystovirus phi6
E634Q site-directed mutagenesis in the specificity pocket, the mutation to glutamine E634 eliminates this salt bridge without major disruption of the internal packaging within the polymerase Cystovirus phi6
E652Q site-directed mutagenesis in the plough region Cystovirus phi6
K147L site-directed mutagenesis in the rim of the template tunnel Cystovirus phi6
K451A site-directed mutagenesis in the specificity pocket Cystovirus phi6
K451L site-directed mutagenesis in the specificity pocket Cystovirus phi6
K541A site-directed mutagenesis in the rim of the template tunnel, the mutation reduces both the positive charge as well as the size and shape of the template tunnel rim Cystovirus phi6
K541L site-directed mutagenesis in the rim of the template tunnel, the mutation reduces both the positive charge as well as the size and shape of the template tunnel rim Cystovirus phi6
K627A site-directed mutagenesis in the rim of the template tunnel Cystovirus phi6
K627L site-directed mutagenesis in the rim of the template tunnel Cystovirus phi6
L628S site-directed mutagenesis in the specificity pocket Cystovirus phi6
additional information structure-based mutagenesis of the enzyme, the elongation rates are not compromised by the mutations, while the transcription rates vary, phenotypes, overview Cystovirus phi6
R146L site-directed mutagenesis in the rim of the template tunnel Cystovirus phi6
R291K site-directed mutagenesis in the specificity pocket Cystovirus phi6
R291S site-directed mutagenesis in the specificity pocket Cystovirus phi6
R30A site-directed mutagenesis in the rim of the template tunnel, the mutation reduces both the positive charge as well as the size and shape of the template tunnel rim Cystovirus phi6
T633A site-directed mutagenesis in the specificity pocket Cystovirus phi6

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nucleoside triphosphate + RNAn Cystovirus phi6
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diphosphate + RNAn+1
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?

Organism

Organism UniProt Comment Textmining
Cystovirus phi6
-
a dsRNA bacteriophage of the family Cystoviridae
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information In silico template RNA modelling, overview Cystovirus phi6 ?
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?
nucleoside triphosphate + RNAn
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Cystovirus phi6 diphosphate + RNAn+1
-
?
nucleoside triphosphate + RNAn NTPs are ATP, GTP, CTP, and UTP, roles of the negatively charged plough area on the polymerase surface, of the rim of the template tunnel and of the template specificity pocket that is key in the formation of the productive RNA-polymerase binary complex. The positively charged rim of the template tunnel has a significant role in the engagement of highly structured ssRNA molecules, whereas specific interactions further down in the template tunnel promote ssRNA entry to the catalytic site Cystovirus phi6 diphosphate + RNAn+1
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?

Subunits

Subunits Comment Organism
More structure-function analysis, molecular mapping, overview Cystovirus phi6

Synonyms

Synonyms Comment Organism
RDRP
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Cystovirus phi6
RNA-dependent RNA polymerase
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Cystovirus phi6