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Literature summary for 2.7.7.48 extracted from

  • Gruez, A.; Selisko, B.; Roberts, M.; Bricogne, G.; Bussetta, C.; Jabafi, I.; Coutard, B.; De Palma, A.M.; Neyts, J.; Canard, B.
    The crystal structure of coxsackievirus B3 RNA-dependent RNA polymerase in complex with its protein primer VPg confirms the existence of a second VPg binding site on Picornaviridae polymerases (2008), J. Virol., 82, 9577-9590.
    View publication on PubMedView publication on EuropePMC
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Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with its protein primer VPg and diphosphate, hanging drop vapour diffusion method, in 20 mM Tris (pH 9.0), 300 mM NaCl, 15% glycerol, 0.5 mM Tris(2-carboxyethyl)phosphine Coxsackievirus B3

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ optimum concentration at 0.8 mM Coxsackievirus B3
Mn2+ optimum concentration at 0.1 mM, RdRp is twice as active at the Mn2+ concentration optimum compared to that of Mg2+ Coxsackievirus B3

Organism

Organism UniProt Comment Textmining
Coxsackievirus B3
-
-
-
Coxsackievirus B3 Nancy
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nucleoside triphosphate + RNAn using poly(rA)/(dT)15 as a template-primer system Coxsackievirus B3 diphosphate + RNAn+1
-
 ?
nucleoside triphosphate + RNAn using poly(rA)/(dT)15 as a template-primer system Coxsackievirus B3 Nancy diphosphate + RNAn+1
-
 ?

Synonyms

Synonyms Comment Organism
3Dpol
-
 Coxsackievirus B3
RDRP
-
 Coxsackievirus B3
RNA-dependent RNA polymerase
-
 Coxsackievirus B3