Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21(DE3) | Hepacivirus C |
Protein Variants | Comment | Organism |
---|---|---|
A105S | 62% activity compared to the wild type enzyme | Hepacivirus C |
A97S | 88% activity compared to the wild type enzyme | Hepacivirus C |
D318N | no activity | Hepacivirus C |
F101A | 89% activity compared to the wild type enzyme | Hepacivirus C |
F101Y | 134% activity compared to the wild type enzyme | Hepacivirus C |
G102A | 32% activity compared to the wild type enzyme | Hepacivirus C |
G104A | 146% activity compared to the wild type enzyme | Hepacivirus C |
H95S | 104% activity compared to the wild type enzyme | Hepacivirus C |
K100I | 20% activity compared to the wild type enzyme | Hepacivirus C |
P93A | 90% activity compared to the wild type enzyme | Hepacivirus C |
P94A | 115% activity compared to the wild type enzyme | Hepacivirus C |
R98I | 68% activity compared to the wild type enzyme | Hepacivirus C |
R98K | 157% activity compared to the wild type enzyme | Hepacivirus C |
S96T | 82% activity compared to the wild type enzyme | Hepacivirus C |
S99T | no activity | Hepacivirus C |
T92S | 18% activity compared to the wild type enzyme | Hepacivirus C |
Y103F | 89% activity compared to the wild type enzyme | Hepacivirus C |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | mAbs 8B2 and 7G8 inhibit the primer-dependent RdRp activity, mAb 8B2 inhibits the elongation of RNA chains, while mAb 7G8 inhibitory effect is achieved through inhibition of multiple RNA synthesis initiations | Hepacivirus C |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hepacivirus C | O92972 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-Sepharose column chromatography | Hepacivirus C |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nucleoside triphosphate + RNAn | poly(C) RNA template | Hepacivirus C | diphosphate + RNAn+1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
nonstructural protein 5B | - |
Hepacivirus C |
NS5B | - |
Hepacivirus C |
RDRP | - |
Hepacivirus C |
RNA-dependent RNA polymerase | - |
Hepacivirus C |