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Literature summary for 2.7.7.48 extracted from

  • Nikonov, A.; Juronen, E.; Ustav, M.
    Functional characterization of fingers subdomain-specific monoclonal antibodies inhibiting the hepatitis C virus RNA-dependent RNA polymerase (2008), J. Biol. Chem., 283, 24089-24102.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21(DE3) Hepacivirus C

Protein Variants

Protein Variants Comment Organism
A105S 62% activity compared to the wild type enzyme Hepacivirus C
A97S 88% activity compared to the wild type enzyme Hepacivirus C
D318N no activity Hepacivirus C
F101A 89% activity compared to the wild type enzyme Hepacivirus C
F101Y 134% activity compared to the wild type enzyme Hepacivirus C
G102A 32% activity compared to the wild type enzyme Hepacivirus C
G104A 146% activity compared to the wild type enzyme Hepacivirus C
H95S 104% activity compared to the wild type enzyme Hepacivirus C
K100I 20% activity compared to the wild type enzyme Hepacivirus C
P93A 90% activity compared to the wild type enzyme Hepacivirus C
P94A 115% activity compared to the wild type enzyme Hepacivirus C
R98I 68% activity compared to the wild type enzyme Hepacivirus C
R98K 157% activity compared to the wild type enzyme Hepacivirus C
S96T 82% activity compared to the wild type enzyme Hepacivirus C
S99T no activity Hepacivirus C
T92S 18% activity compared to the wild type enzyme Hepacivirus C
Y103F 89% activity compared to the wild type enzyme Hepacivirus C

Inhibitors

Inhibitors Comment Organism Structure
additional information mAbs 8B2 and 7G8 inhibit the primer-dependent RdRp activity, mAb 8B2 inhibits the elongation of RNA chains, while mAb 7G8 inhibitory effect is achieved through inhibition of multiple RNA synthesis initiations Hepacivirus C

Organism

Organism UniProt Comment Textmining
Hepacivirus C O92972
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-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-Sepharose column chromatography Hepacivirus C

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nucleoside triphosphate + RNAn poly(C) RNA template Hepacivirus C diphosphate + RNAn+1
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Synonyms

Synonyms Comment Organism
nonstructural protein 5B
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Hepacivirus C
NS5B
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Hepacivirus C
RDRP
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Hepacivirus C
RNA-dependent RNA polymerase
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Hepacivirus C