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Literature summary for 2.7.7.48 extracted from

  • Yu, F.; Hasebe, F.; Inoue, S.; Mathenge, E.G.; Morita, K.
    Identification and characterization of RNA-dependent RNA polymerase activity in recombinant Japanese encephalitis virus NS5 protein (2007), Arch. Virol., 152, 1859-1869.
    View publication on PubMed
Search for more literature for 2.7.7.48

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli XL1 Blue cells Japanese encephalitis virus
expression of wild-type and mutant enzymes in Escherichia coli Japanese encephalitis virus

Protein Variants

Protein Variants Comment Organism
D536A inactive Japanese encephalitis virus
D536A completely inactive enzyme Japanese encephalitis virus
D668A inactive Japanese encephalitis virus
D668A completely inactive enzyme Japanese encephalitis virus
D669A inactive Japanese encephalitis virus
D669A completely inactive enzyme Japanese encephalitis virus
D669N reduction in activity to about 5% relative to the parental NS5 Japanese encephalitis virus
D669N mutant shows a reduction in activity to about 5% relative to the wild type enzyme Japanese encephalitis virus
G605A inactive Japanese encephalitis virus
G605A completely inactive enzyme Japanese encephalitis virus
K691A inactive Japanese encephalitis virus
K691A completely inactive enzyme Japanese encephalitis virus

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ no activity at 3 mM Japanese encephalitis virus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent on divalent cations, Mn2+ is 20times more effective than Mg2+ at optimal concentration (3 mM) Japanese encephalitis virus
Mg2+ Mg2+ is 20 times less effective than Mn2+ in coordinating the catalytic reaction of RdRp Japanese encephalitis virus
Mn2+ dependent on divalent cations, Mn2+ is 20times more effective than Mg2+ at optimal concentration (3 mM) Japanese encephalitis virus
Mn2+ Mn2+ is 20 times more effective than Mg2+ in coordinating the catalytic reaction of RdRp Japanese encephalitis virus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
103000
-
 SDS-PAGE Japanese encephalitis virus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nucleoside triphosphate + RNAn Japanese encephalitis virus JEV NS5 protein can initiate RNA synthesis through a de novo initiation mechanism. JEV NS5 protein is more efficient in using negative-strand RNA templates, indicating that the JEV NS5 protein is involved in regulating the ratio of positive strand RNA to negative strand RNA diphosphate + RNAn+1
-
 ?
nucleoside triphosphate + RNAn Japanese encephalitis virus JaOH0566 JEV NS5 protein can initiate RNA synthesis through a de novo initiation mechanism. JEV NS5 protein is more efficient in using negative-strand RNA templates, indicating that the JEV NS5 protein is involved in regulating the ratio of positive strand RNA to negative strand RNA diphosphate + RNAn+1
-
 ?

Organism

Organism UniProt Comment Textmining
Japanese encephalitis virus
-
-
-
Japanese encephalitis virus JaOH0566
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Japanese encephalitis virus
Talon IMAC resin column chromatography Japanese encephalitis virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nucleoside triphosphate + RNAn JEV NS5 protein can initiate RNA synthesis through a de novo initiation mechanism. JEV NS5 protein is more efficient in using negative-strand RNA templates, indicating that the JEV NS5 protein is involved in regulating the ratio of positive strand RNA to negative strand RNA Japanese encephalitis virus diphosphate + RNAn+1
-
 ?
nucleoside triphosphate + RNAn JEV NS5 protein can initiate RNA synthesis through a de novo initiation mechanism. JEV NS5 protein is more efficient in using negative-strand RNA templates Japanese encephalitis virus diphosphate + RNAn+1
-
 ?
nucleoside triphosphate + RNAn uses JEV and dengue-2 virus 3' end plus- and minus-strand RNA templates, the incorporation of [32P]-UMP is much lower when using positive-strand RNA as template than when using negative-strand RNA - an almost 10fold difference in efficiency Japanese encephalitis virus diphosphate + RNAn+1
-
 ?
nucleoside triphosphate + RNAn JEV NS5 protein can initiate RNA synthesis through a de novo initiation mechanism. JEV NS5 protein is more efficient in using negative-strand RNA templates, indicating that the JEV NS5 protein is involved in regulating the ratio of positive strand RNA to negative strand RNA Japanese encephalitis virus JaOH0566 diphosphate + RNAn+1
-
 ?
nucleoside triphosphate + RNAn JEV NS5 protein can initiate RNA synthesis through a de novo initiation mechanism. JEV NS5 protein is more efficient in using negative-strand RNA templates Japanese encephalitis virus JaOH0566 diphosphate + RNAn+1
-
 ?
nucleoside triphosphate + RNAn uses JEV and dengue-2 virus 3' end plus- and minus-strand RNA templates, the incorporation of [32P]-UMP is much lower when using positive-strand RNA as template than when using negative-strand RNA - an almost 10fold difference in efficiency Japanese encephalitis virus JaOH0566 diphosphate + RNAn+1
-
 ?

Subunits

Subunits Comment Organism
? x * 103000, SDS-PAGE Japanese encephalitis virus

Synonyms

Synonyms Comment Organism
JEV NS5 protein
-
 Japanese encephalitis virus
NS5 protein
-
 Japanese encephalitis virus
RDRP
-
 Japanese encephalitis virus
RNA-dependent RNA polymerase
-
 Japanese encephalitis virus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Japanese encephalitis virus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 40 25°C: about 55% of maximal activity, 40°C: about 20% of maximal activity Japanese encephalitis virus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8
-
 Japanese encephalitis virus

pH Range

pH Minimum pH Maximum Comment Organism
6 9 pH 6: about 10% of maximal activity, pH 9: about 20% of maximal activity Japanese encephalitis virus