Literature summary for 2.7.7.48 extracted from

Carroll, S.S.; Sardana, V.; Yang, Z.; Jacobs, A.R.; Mizenko, C.; Hall, D.; Hill, L.; Zugay-Murphy, J.; Kuo, L.C.
Only a small fraction of purified hepatitis C RNA-dependent RNA polymerase is catalytically competent: implications for viral replication and in vitro assays (2000), Biochemistry, 39, 8243-8249.

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KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0006	-	CTP	pH 7.5, 23°C, reaction with heteropolymeric template T500, C-terminally truncated enzyme form NS5B(DELTA21)	Hepacivirus C
0.0006	-	UTP	pH 7.5, 23°C, reaction with heteropolymeric template T500, C-terminally truncated enzyme form NS5B(DELTA21)	Hepacivirus C
0.0014	-	GTP	pH 7.5, 23°C, reaction with heteropolymeric template T500, C-terminally truncated enzyme form NS5B(DELTA21)	Hepacivirus C
0.0029	-	ATP	pH 7.5, 23°C, reaction with heteropolymeric template T500, C-terminally truncated enzyme form NS5B(DELTA21)	Hepacivirus C
0.0057	-	UTP	pH 7.5, 23°C, C-terminally truncated enzyme form NS5B(DELTA21), reaction with dT16/poly(rA)	Hepacivirus C
0.013	-	UTP	pH 7.5, 23°C, full-length enzyme, reaction with dT16/poly(rA)	Hepacivirus C

Organism

Organism	UniProt	Comment	Textmining
Hepacivirus C	-	expressed in Escherichia coli	-

Purification (Commentary)

Purification (Comment)	Organism
C-terminally truncated enzyme form NS5B(DELTA21) expressed in Escherichia coli	Hepacivirus C

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.025	-	truncated enzyme form NS5B(DELTA21)	Hepacivirus C

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + RNAn	-	Hepacivirus C	diphosphate + RNAn+1	-	?
CTP + RNAn	-	Hepacivirus C	diphosphate + RNAn+1	-	?
GTP + RNAn	-	Hepacivirus C	diphosphate + RNAn+1	-	?
nucleoside triphosphate + RNAn	once synthesis has begun, the C-terminally truncated enzyme NS5B(DELTA21) does not dissociate from the template until a complete double strand copy of the RNA is made	Hepacivirus C	diphosphate + RNAn+1	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.000105	-	ATP	pH 7.5, 23°C, reaction with heteropolymeric template T500, C-terminally truncated enzyme form NS5B(DELTA21)	Hepacivirus C
0.000133	-	CTP	pH 7.5, 23°C, reaction with heteropolymeric template T500, C-terminally truncated enzyme form NS5B(DELTA21)	Hepacivirus C
0.000167	-	UTP	pH 7.5, 23°C, reaction with heteropolymeric template T500, C-terminally truncated enzyme form NS5B(DELTA21)	Hepacivirus C
0.000383	-	GTP	pH 7.5, 23°C, reaction with heteropolymeric template T500, C-terminally truncated enzyme form NS5B(DELTA21)	Hepacivirus C
0.00167	-	UTP	pH 7.5, 23°C, full-length enzyme, reaction with dT16/poly(rA)	Hepacivirus C
0.0272	-	UTP	pH 7.5, 23°C, C-terminally truncated enzyme form NS5B(DELTA21), reaction with dT16/poly(rA)	Hepacivirus C

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Release 2023.1 (January 2023)