Protein Variants | Comment | Organism |
---|---|---|
D238A | poly(rU) polymerase activity determined by using dT15/rA30 in presence of Mn2+ is 45% of the activity of the wild-type enzyme. AMP incorporation in presence of Mg2+, at 0.1 mM ATP, is 0.24% of the activity of the wild-type enzyme. AMP incorporation in presence of Mn2+, at 0.1 mM ATP, is 1.9% of the activity of the wild-type enzyme | Enterovirus C |
D238E | poly(rU) polymerase activity determined by using dT15/rA30 in presence of Mn2+ is 45% of the activity of the wild-type enzyme. AMP incorporation in presence of Mg2+, at 0.1 mM ATP, is 0.04% of the activity of the wild-type enzyme. AMP incorporation in presence of Mn2+, at 0.1 mM ATP, is 0.74% of the activity of the wild-type enzyme | Enterovirus C |
D238F | poly(rU) polymerase activity determined by using dT15/rA30 in presence of Mn2+ is 4.4% of the activity of the wild-type enzyme. No AMP incorporation activity in presence of Mn2+ or Mg2+ | Enterovirus C |
D238N | poly(rU) polymerase activity determined by using dT15/rA30 in presence of Mn2+ is 45% of the activity of the wild-type enzyme. AMP incorporation in presence of Mg2+, at 0.1 mM ATP, is 0.05% of the activity of the wild-type enzyme. AMP incorporation in presence of Mn2+, at 0.1 mM ATP, is 0.797% of the activity of the wild-type enzyme | Enterovirus C |
D238V | poly(rU) polymerase activity determined by using dT15/rA30 in presence of Mn2+ is 11% of the activity of the wild-type enzyme. No AMP incorporation activity in presence of Mn2+ or Mg2+ | Enterovirus C |
N297A | poly(rU) polymerase activity determined by using dT15/rA30 in presence of Mn2+ is 89% of the activity of the wild-type enzyme. AMP incorporation in presence of Mg2+, at 0.1 mM ATP, is 11.2% of the activity of the wild-type enzyme. AMP incorporation in presence of Mn2+, at 0.1 mM ATP, is 4.5% of the activity of the wild-type enzyme | Enterovirus C |
N297D | poly(rU) polymerase activity determined by using dT15/rA30 in presence of Mn2+ is 89% of the activity of the wild-type enzyme. AMP incorporation in presence of Mg2+, at 0.1 mM ATP, is 38.2% of the activity of the wild-type enzyme. AMP incorporation in presence of Mn2+, at 0.1 mM ATP, is 21.2% of the activity of the wild-type enzyme | Enterovirus C |
N297Q | poly(rU) polymerase activity determined by using dT15/rA30 in presence of Mn2+ is about 35% of the activity of the wild-type enzyme. AMP incorporation in presence of Mg2+, at 0.1 mM ATP, is 1.4% of the activity of the wild-type enzyme. AMP incorporation in presence of Mn2+, at 0.1 mM ATP, is 0.72% of the activity of the wild-type enzyme | Enterovirus C |
N297V | poly(rU) polymerase activity determined by using dT15/rA30 in presence of Mn2+ is about 55% of the activity of the wild-type enzyme. AMP incorporation in presence of Mg2+, at 0.1 mM ATP, is 15.3% of the activity of the wild-type enzyme. AMP incorporation in presence of Mn2+, at 0.1 mM ATP, is 4.9% of the activity of the wild-type enzyme | Enterovirus C |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | stimulates | Enterovirus C | |
Mn2+ | stimulates to a higher extent than Mg2+, poly(rU) polymerase activity using dT15/rA30 | Enterovirus C |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterovirus C | - |
expressed in Escherichia coli | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nucleoside triphosphate + RNAn | - |
Enterovirus C | diphosphate + RNAn+1 | - |
? |