Protein Variants | Comment | Organism |
---|---|---|
C274A | mutation completely abolishes RNA-dependent RNA polymerase activity | Hepacivirus C |
E18A | mutation completely abolishes RNA-dependent RNA polymerase activity | Hepacivirus C |
H502A | mutation completely abolishes RNA-dependent RNA polymerase activity | Hepacivirus C |
additional information | mutant cm20t in which 7 amino acids in a row are changed to AAASAAA from aa17-23, is totally defective in RNA-dependent RNA polymerase activity | Hepacivirus C |
additional information | mutant cm1940t in which 7 amino acids in a row are changed to AAASAAA from aa191-197, is totally defective in RNA-dependent RNA polymerase activity | Hepacivirus C |
additional information | mutant enzymes cm2t and cm3t are totally defective in RNA-dependent RNA polymerase activity | Hepacivirus C |
additional information | mutant enzyme cm223t in which 7 amino acids in a row are changed to AAASAAA from aa220-226, shows 50% of the activity of the wild-type enzyme | Hepacivirus C |
Y191A | mutation completely abolishes RNA-dependent RNA polymerase activity | Hepacivirus C |
Y276A | mutation completely abolishes RNA-dependent RNA polymerase activity | Hepacivirus C |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hepacivirus C | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, wild-type and mutant enzymes, expressed in Escherichia coli | Hepacivirus C |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nucleoside triphosphate + RNAn | - |
Hepacivirus C | diphosphate + RNAn+1 | - |
? |