Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cl- | - |
black beetle virus | |
Mn2+ | above 2 mM | black beetle virus | |
RNase | pancreatic RNAse and T1 RNAse | black beetle virus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
UTP | pH 8.2, 29°C | black beetle virus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | particulate material of cells of Drosophila melanogaster infected with Black beetle virus | black beetle virus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | slight stimulation by potassium acetate, optimal activity at 60 mM | black beetle virus | |
Mg2+ | required, optimal activity with 10-20 mM magnesium acetate | black beetle virus | |
Mn2+ | Mg2+ requirement can be partially replaced by Mn2+ | black beetle virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
black beetle virus | - |
Drosophila melanogaster infected with | - |
Purification (Comment) | Organism |
---|---|
- |
black beetle virus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | cells of Drosophila melanogaster infected with Black beetle virus | black beetle virus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00000016 | - |
- |
black beetle virus |
Storage Stability | Organism |
---|---|
-70°C, stable for several months | black beetle virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nucleoside triphosphate + RNAn | full-length negative strand BBV RNAs are synthesized | black beetle virus | diphosphate + RNAn+1 | - |
? | |
UTP + RNAn | - |
black beetle virus | diphosphate + RNAn+1 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
29 | - |
- |
black beetle virus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
18 | 37 | 18°C: about 50% of maximal activity, 37°C: about 50% of maximal activity | black beetle virus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
- |
black beetle virus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.6 | 9 | pH 7.6: about 70% of maximal activity, pH 9.0: about 80% of maximal activity | black beetle virus |