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Literature summary for 2.7.7.48 extracted from

  • Blumenthal, T.
    Interaction of Qbeta RNA replicase with guanine nucleotides. Different modes of inhibition and inactivation (1977), Biochim. Biophys. Acta, 478, 201-208.
    View publication on PubMed
Search for more literature for 2.7.7.48

Inhibitors

Inhibitors Comment Organism Structure
GDP competitive with GTP Qubevirus durum
ppGpp competitive with GTP Qubevirus durum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
 GTP 30°C Qubevirus durum
0.02
-
 GTP 30°C, cross-linked enzyme Qubevirus durum

Organic Solvent Stability

Organic Solvent Comment Organism
urea denaturation Qubevirus durum

Organism

Organism UniProt Comment Textmining
Qubevirus durum
-
-
-

Renatured (Commentary)

Renatured (Comment) Organism
when the enzyme is denatured with 8 M urea, the rate of its renaturation following dilution in a high-salt buffer is inhibited by relatively low concentrations of GDP, ppGpp and GTP Qubevirus durum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + RNAn
-
 Qubevirus durum diphosphate + RNAn+1
-
 ?
nucleoside triphosphate + RNAn
-
 Qubevirus durum diphosphate + RNAn+1
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 60 min, 40-60% loss of poly(G) polymerase activity, in presence of 2 mM guanine nucleotides loss of activity is up to 91% of the original specific activity Qubevirus durum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.1
-
 GDP 30°C Qubevirus durum
0.25
-
 ppGpp 30°C Qubevirus durum