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Literature summary for 2.7.7.46 extracted from

  • Van Pelt, J.E.; Northrop, D.B.
    Purification and properties of gentamicin nucleotidyltransferase from Escherichia coli: nucleotide specificity, pH optimum, and the separation of two electrophoretic variants (1984), Arch. Biochem. Biophys., 230, 250-263.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
MgATP2- metal chelates being the true substrates, free nucleotides being inhibitors Escherichia coli
MgCTP2- metal chelates being the true substrates, free nucleotides being inhibitors Escherichia coli
MgdATP2- metal chelates being the true substrates, free nucleotides being inhibitors Escherichia coli
MgdCTP2- metal chelates being the true substrates, free nucleotides being inhibitors Escherichia coli
MgdGTP2- metal chelates being the true substrates, free nucleotides being inhibitors Escherichia coli
MgdTTP2- metal chelates being the true substrates, free nucleotides being inhibitors Escherichia coli
MgGTP2- metal chelates being the true substrates, free nucleotides being inhibitors Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00276
-
tobramycin pH 9.0, 25°C Escherichia coli
0.03
-
dATP pH 9.0, 25°C Escherichia coli
0.03
-
dGTP pH 9.0, 25°C Escherichia coli
0.03
-
dATP dATP in form of MgdATP2- Escherichia coli
0.03
-
dGTP dGTP in form of MgdGTP2- Escherichia coli
0.09
-
dTTP pH 9.0, 25°C Escherichia coli
0.09
-
dCTP pH 9.0, 25°C Escherichia coli
0.09
-
dTTP dTTP in form of MgdTTP2- Escherichia coli
0.09
-
dCTP dCTP in form of MgdCTP2- Escherichia coli
0.404
-
ATP pH 9.0, 25°C Escherichia coli
0.404
-
ATP ATP in form of MgATP2- Escherichia coli
2.008
-
CTP pH 9.0, 25°C Escherichia coli
2.008
-
CTP CTP in form of MgCTP2- Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31500
-
isoform II, SDS-PAGE Escherichia coli
32500
-
isoform I, SDS-PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
2 related isoforms Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.134
-
isoform I Escherichia coli
0.274
-
isoform II Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + tobramycin
-
Escherichia coli diphosphate + 2''-adenylyltobramycin
-
r
CTP + tobramycin
-
Escherichia coli diphosphate + 2''-cytosylyltobramycin
-
r
dATP + tobramycin
-
Escherichia coli diphosphate + 2''-deoxyadenylyltobramycin
-
r
dCTP + tobramycin
-
Escherichia coli diphosphate + 2''-deoxycytosylyltobramycin
-
r
dGTP + tobramycin
-
Escherichia coli diphosphate + 2''-deoxyguanosylyltobramycin
-
r
dTTP + tobramycin
-
Escherichia coli diphosphate + 2''-deoxythymidylyltobramycin
-
r
GTP + tobramycin
-
Escherichia coli diphosphate + 2''-guanosylyltobramycin
-
r

Subunits

Subunits Comment Organism
monomer 1 * 32500, isoform I, SDS-PAGE Escherichia coli
monomer 1 * 31500, isoform II, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
AAD 2''
-
Escherichia coli
ANT2''
-
Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
isoform I Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
6.5 10
-
Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.01
-
MgGTP2- pH 9.0, 25°C Escherichia coli
0.011
-
MgdATP2- pH 9.0, 25°C Escherichia coli
0.027
-
MgdGTP pH 9.0, 25°C Escherichia coli
0.047
-
MgdCTP pH 9.0, 25°C Escherichia coli
0.145
-
MgdTTP pH 9.0, 25°C Escherichia coli
0.404
-
MgCTP pH 9.0, 25°C Escherichia coli
0.864
-
MgATP2- pH 9.0, 25°C Escherichia coli

pI Value

Organism Comment pI Value Maximum pI Value
Escherichia coli 2 related isoforms, isoelectric focusing
-
5.7
Escherichia coli 2 related isoforms, isoelectric focusing
-
6