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Literature summary for 2.7.7.43 extracted from

  • Li, Y.; Yu, H.; Cao, H.; Muthana, S.; Chen, X.
    Pasteurella multocida CMP-sialic acid synthetase and mutants of Neisseria meningitidis CMP-sialic acid synthetase with improved substrate promiscuity (2012), Appl. Microbiol. Biotechnol., 93, 2411-2423.
    View publication on PubMed
Search for more literature for 2.7.7.43

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Neisseria meningitidis
expression in Escherichia coli Pasteurella multocida
expression in Escherichia coli [Haemophilus] ducreyi

Crystallization (Commentary)

Crystallization (Comment) Organism
modeling of the closed conformation. Enzyme forms a dimer complex. Amino acids involved in the Neu5Ac binding pocket are Glu162 and Arg165 from monomer B and Gln104, Ser82, Val86, Tyr179, and Phe192 from monomer A Neisseria meningitidis

Protein Variants

Protein Variants Comment Organism
Q163A mutant displays improved substrate promiscuity Neisseria meningitidis
S31R mutant displays improved substrate promiscuity, catalytic activities for substrates N-glycolylneuraminate, N-methylglycolylneuraminate and 8-O-methyl N-acetyl-neuraminate are improved compared to wild-type Neisseria meningitidis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 N-acetylneuraminate mutant S81R, pH 8.5, 37°C Neisseria meningitidis
0.14
-
 N-acetylneuraminate wild-type, pH 8.5, 37°C Pasteurella multocida
0.16
-
 CTP wild-type, pH 8.5, 37°C [Haemophilus] ducreyi
0.18
-
 N-acetylneuraminate wild-type, pH 8.5, 37°C [Haemophilus] ducreyi
0.22
-
 N-acetylneuraminate wild-type, pH 8.5, 37°C Neisseria meningitidis
0.22
-
 CTP mutant S81R, pH 8.5, 37°C Neisseria meningitidis
0.24
-
 CTP wild-type, pH 8.5, 37°C Pasteurella multocida
0.59
-
 CTP wild-type, pH 8.5, 37°C Neisseria meningitidis
1.5
-
 8-O-methyl-N-acetylneuraminate mutant S81R, pH 8.5, 37°C Neisseria meningitidis
2
-
 N-methylglycolylneuraminate wild-type, pH 8.5, 37°C Neisseria meningitidis
4
-
 N-glycolylneuraminate mutant S81R, pH 8.5, 37°C Neisseria meningitidis
5.3
-
 8-O-methyl-N-acetylneuraminate wild-type, pH 8.5, 37°C Neisseria meningitidis
6.2
-
 N-glycolylneuraminate wild-type, pH 8.5, 37°C Neisseria meningitidis
7
-
 N-methylglycolylneuraminate mutant S81R, pH 8.5, 37°C Neisseria meningitidis

Organism

Organism UniProt Comment Textmining
Neisseria meningitidis
-
-
-
Pasteurella multocida
-
-
-
Pasteurella multocida ATCC 15742
-
-
-
[Haemophilus] ducreyi
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + 8-O-methyl-N-acetylneuraminate
-
 Neisseria meningitidis diphosphate + CMP-(8-O-methyl)-N-acetylneuraminate
-
 ?
CTP + N-acetylneuraminate
-
 Neisseria meningitidis diphosphate + CMP-N-acetylneuraminate
-
 ?
CTP + N-acetylneuraminate
-
 Pasteurella multocida diphosphate + CMP-N-acetylneuraminate
-
 ?
CTP + N-acetylneuraminate
-
 [Haemophilus] ducreyi diphosphate + CMP-N-acetylneuraminate
-
 ?
CTP + N-acetylneuraminate
-
 Pasteurella multocida ATCC 15742 diphosphate + CMP-N-acetylneuraminate
-
 ?
CTP + N-glycolylneuraminate
-
 Neisseria meningitidis diphosphate + CMP-N-glycolylneuraminate
-
 ?
CTP + N-methylglycolylneuraminate
-
 Neisseria meningitidis diphosphate + CMP-N-methylglycolylneuraminate
-
 ?

Synonyms

Synonyms Comment Organism
CSS
-
 Neisseria meningitidis
CSS
-
 Pasteurella multocida
CSS
-
 [Haemophilus] ducreyi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.9
-
 8-O-methyl-N-acetylneuraminate mutant S81R, pH 8.5, 37°C Neisseria meningitidis
2.9
-
 N-methylglycolylneuraminate wild-type, pH 8.5, 37°C Neisseria meningitidis
3.2
-
 N-glycolylneuraminate wild-type, pH 8.5, 37°C Neisseria meningitidis
4.2
-
 N-acetylneuraminate wild-type, pH 8.5, 37°C Pasteurella multocida
4.6
-
 CTP wild-type, pH 8.5, 37°C Pasteurella multocida
4.8
-
 N-acetylneuraminate wild-type, pH 8.5, 37°C [Haemophilus] ducreyi
5.2
-
 CTP wild-type, pH 8.5, 37°C [Haemophilus] ducreyi
7.4
-
 N-acetylneuraminate mutant S81R, pH 8.5, 37°C Neisseria meningitidis
8.8
-
 CTP mutant S81R, pH 8.5, 37°C Neisseria meningitidis
12
-
 N-methylglycolylneuraminate mutant S81R, pH 8.5, 37°C Neisseria meningitidis
19
-
 N-acetylneuraminate wild-type, pH 8.5, 37°C Neisseria meningitidis
21
-
 CTP wild-type, pH 8.5, 37°C Neisseria meningitidis
21
-
 8-O-methyl-N-acetylneuraminate wild-type, pH 8.5, 37°C Neisseria meningitidis
23
-
 N-glycolylneuraminate mutant S81R, pH 8.5, 37°C Neisseria meningitidis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Neisseria meningitidis
8.5
-
-
 Pasteurella multocida
8.5
-
-
 [Haemophilus] ducreyi

pH Range

pH Minimum pH Maximum Comment Organism
7.5 9
-
 Neisseria meningitidis
7.5 9
-
 Pasteurella multocida
7.5 9
-
 [Haemophilus] ducreyi

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.55
-
 8-O-methyl-N-acetylneuraminate wild-type, pH 8.5, 37°C Neisseria meningitidis
1.3
-
 8-O-methyl-N-acetylneuraminate mutant S81R, pH 8.5, 37°C Neisseria meningitidis
1.6
-
 N-methylglycolylneuraminate wild-type, pH 8.5, 37°C Neisseria meningitidis
1.7
-
 N-methylglycolylneuraminate mutant S81R, pH 8.5, 37°C Neisseria meningitidis
3.8
-
 N-glycolylneuraminate wild-type, pH 8.5, 37°C Neisseria meningitidis
5.9
-
 N-glycolylneuraminate mutant S81R, pH 8.5, 37°C Neisseria meningitidis
19
-
 CTP wild-type, pH 8.5, 37°C Pasteurella multocida
27
-
 N-acetylneuraminate wild-type, pH 8.5, 37°C [Haemophilus] ducreyi
30
-
 N-acetylneuraminate wild-type, pH 8.5, 37°C Pasteurella multocida
33
-
 CTP wild-type, pH 8.5, 37°C [Haemophilus] ducreyi
36
-
 CTP wild-type, pH 8.5, 37°C Neisseria meningitidis
40
-
 CTP mutant S81R, pH 8.5, 37°C Neisseria meningitidis
74
-
 N-acetylneuraminate mutant S81R, pH 8.5, 37°C Neisseria meningitidis
86
-
 N-acetylneuraminate wild-type, pH 8.5, 37°C Neisseria meningitidis