Literature summary for 2.7.7.43 extracted from

Feo-Manga, J.C.; Rodriguez-Aparicio, L.B.; Ferrero, M.A.; Reglero, A.
Purification and partial characterization of CMP-Neu5Ac synthetase from rat brain (2006), Anal. Chim. Acta, 564, 141-150.

No PubMed abstract available

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Inhibitors

Inhibitors	Comment	Organism	Structure
Co2+	-	Rattus norvegicus
Cu2+	-	Rattus norvegicus
DTT	in the presence of 0.01 M DTT enzyme shows 20% activity loss	Rattus norvegicus
Hg2+	-	Rattus norvegicus
Mn2+	-	Rattus norvegicus
additional information	enzyme is inhibited by thiol-modifying reagents; enzyme is not inhibited by thiol-containing reagents like GSH, mercaptoethanol and cysteine	Rattus norvegicus
NaF	in the presence of 0.06 M NaF enzyme shows 17% activity loss	Rattus norvegicus
Sn2+	-	Rattus norvegicus
Zn2+	-	Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
46000	-	-	Rattus norvegicus

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
by column chromatography on Sephacryl S-200 (gel filtration), Yellow-86-Agarose (affinity) and Phenyl-Sepharose (hydrophobic affinity)	Rattus norvegicus

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	-	Rattus norvegicus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
CTP + N-acetyl-neuraminic acid	-	Rattus norvegicus	CMP-Neu5Ac + diphosphate	-	?

Subunits

Subunits	Comment	Organism
tetramer	4*190000, gel filtration	Rattus norvegicus

Synonyms

Synonyms	Comment	Organism
CMP-Neu5Ac synthetase	-	Rattus norvegicus
N-Acetyl-neuraminic acid cytidylyltransferase	-	Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	greatest activity is observed at 40°C	Rattus norvegicus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	-	-	Rattus norvegicus

pH Range

pH Minimum	pH Maximum	Comment	Organism
8.5	9.5	-	Rattus norvegicus

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Release 2023.1 (January 2023)