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Literature summary for 2.7.7.43 extracted from

  • Fujita, A.; Sato, C.; Munster-Kuhnel, A.K.; Gerardy-Schahn, R.; Kitajima, K.
    Development of a simple and efficient method for assaying cytidine monophosphate sialic acid synthetase activity using an enzymatic reduced nicotinamide adenine dinucleotide/oxidized nicotinamide adenine dinucleotide converting system (2005), Anal. Biochem., 337, 12-21.
    View publication on PubMed
Search for more literature for 2.7.7.43

Protein Variants

Protein Variants Comment Organism
K198A mutant enzyme is active at moderately reduced level Mus musculus
R199A inactive mutant protein Mus musculus
R199A/R202A inactive mutant protein Mus musculus
R201A mutant enzyme is active at moderately reduced level Mus musculus
R202A mutant enzyme shows drastically reduced activity Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
 recombinant
-
Oncorhynchus mykiss
-
 recombinant
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 simple and efficient method for assaying cytidine monophosphate sialic acid synthetase activity using an enzymatic reduced nicotinamide adenine dinucleotide/oxidized nicotinamide adenine dinucleotide converting system Mus musculus
additional information
-
 simple and efficient method for assaying cytidine monophosphate sialic acid synthetase activity using an enzymatic reduced nicotinamide adenine dinucleotide/oxidized nicotinamide adenine dinucleotide converting system Oncorhynchus mykiss

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + deaminoneuraminic acid
-
 Oncorhynchus mykiss ?
-
 ?
CTP + N-acylneuraminate enzyme is equally active with either of the three substrates: N-acylneuraminate, N-glycolylneuraminate and deaminoneuraminic acid Oncorhynchus mykiss diphosphate + CMP-N-acylneuraminate
-
 ?
CTP + N-acylneuraminate enzyme shows pronounced specificity for N-acylneuraminate Mus musculus diphosphate + CMP-N-acylneuraminate
-
 ?
CTP + N-glycolylneuraminate
-
 Oncorhynchus mykiss diphosphate + CMP-N-glycolylneuraminate
-
 ?

Synonyms

Synonyms Comment Organism
CMP-Sia synthetase
-
 Mus musculus
CMP-Sia synthetase
-
 Oncorhynchus mykiss
CSS
-
 Mus musculus
CSS
-
 Oncorhynchus mykiss
cytidine monophosphate sialic acid synthetase
-
 Mus musculus
cytidine monophosphate sialic acid synthetase
-
 Oncorhynchus mykiss