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Literature summary for 2.7.7.41 extracted from

  • Kelley, M.J.; Carman, G.M.
    Purification and characterization of CDP-diacylglycerol synthase from Saccharomyces cerevisiae (1987), J. Biol. Chem., 262, 14563-14570.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 maximal stimulation at 15 mM Saccharomyces cerevisiae

General Stability

General Stability Organism
stable to at least 2 cycles of freezing and thawing Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
dCTP competitive with respect to CTP Saccharomyces cerevisiae
Hg2+ 5 mM HgCl2, 78% inhibition Saccharomyces cerevisiae
PCMB 5 mM, 88% inhibition Saccharomyces cerevisiae
Triton X-100 above 20 mM Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
phosphatidate pH 6.5, 30°C Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrial membrane
-
Saccharomyces cerevisiae 31966
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ maximal activity at 20 mM Mg2+ Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
114000
-
radiation inactivation Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharomyces cerevisiae

Reaction

Reaction Comment Organism Reaction ID
CTP + phosphatidate = diphosphate + CDP-diacylglycerol sequential bi bi reaction mechanism Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.409
-
-
Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
-80°C, in presence of 1 mM CTP the purified enzyme is 90-100% stable for at least 3 months Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + phosphatidate equilibrium constant is 0.001. Reverse reaction is favored in vitro Saccharomyces cerevisiae diphosphate + CDPdiacylglycerol
-
r

Subunits

Subunits Comment Organism
More two polypeptides of 54000 Da and 56000 Da are detected by SDS-PAGE, it is possible that the 54000 Da subunit is a proteolysis product of the 56000 Da subunit Saccharomyces cerevisiae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
20 min, labile above Saccharomyces cerevisiae
60
-
20 min, complete inactivation Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8.92
-
CTP pH 6.5, 30°C Saccharomyces cerevisiae
8.92
-
phosphatidate pH 6.5, 30°C Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
6 8.5 pH 6.0: 70% of maximal activity, pH 8.5: about 70% of maximal activity Saccharomyces cerevisiae