Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | maximal stimulation at 15 mM | Saccharomyces cerevisiae |
General Stability | Organism |
---|---|
stable to at least 2 cycles of freezing and thawing | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dCTP | competitive with respect to CTP | Saccharomyces cerevisiae | |
Hg2+ | 5 mM HgCl2, 78% inhibition | Saccharomyces cerevisiae | |
PCMB | 5 mM, 88% inhibition | Saccharomyces cerevisiae | |
Triton X-100 | above 20 mM | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
phosphatidate | pH 6.5, 30°C | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial membrane | - |
Saccharomyces cerevisiae | 31966 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | maximal activity at 20 mM Mg2+ | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
114000 | - |
radiation inactivation | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
CTP + phosphatidate = diphosphate + CDP-diacylglycerol | sequential bi bi reaction mechanism | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.409 | - |
- |
Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-80°C, in presence of 1 mM CTP the purified enzyme is 90-100% stable for at least 3 months | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + phosphatidate | equilibrium constant is 0.001. Reverse reaction is favored in vitro | Saccharomyces cerevisiae | diphosphate + CDPdiacylglycerol | - |
r |
Subunits | Comment | Organism |
---|---|---|
More | two polypeptides of 54000 Da and 56000 Da are detected by SDS-PAGE, it is possible that the 54000 Da subunit is a proteolysis product of the 56000 Da subunit | Saccharomyces cerevisiae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
20 min, labile above | Saccharomyces cerevisiae |
60 | - |
20 min, complete inactivation | Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.92 | - |
CTP | pH 6.5, 30°C | Saccharomyces cerevisiae | |
8.92 | - |
phosphatidate | pH 6.5, 30°C | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | pH 6.0: 70% of maximal activity, pH 8.5: about 70% of maximal activity | Saccharomyces cerevisiae |