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Literature summary for 2.7.7.41 extracted from
- Purification and properties of the membrane-bound CDP-diglyceride synthetase from Escherichia coli (1985), J. Biol. Chem., 260, 12084-12091.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CTP | - |
Escherichia coli |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.28 | - |
phosphatidic acid | pH 7.4, 30°C | Escherichia coli | |
| 0.58 | - |
dCTP | pH 7.4, 30°C | Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
K12 strain DH1 with plasmid pCD100 causing 53fold overproduction of the enzyme | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| CTP + phosphatidate = diphosphate + CDP-diacylglycerol | sequential mechanism | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 30.79 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CTP + 1,2-dioleoyl phosphatidic acid | reaction with 1-oleoyl-2-palmitoyl-phosphatidic acid at 87.5% of the activity with phosphatidic acid from egg phosphatidylcholine | Escherichia coli | diphosphate + CDP-dioleoylglycerol | - |
? | |
| CTP + 1,2-dipalmitoyl phosphatidic acid | reaction with 1-oleoyl-2-palmitoyl phosphatidic acid at 4.6% of the activity with phosphatidic acid from egg phosphatidylcholine | Escherichia coli | diphosphate + CDP-dipalmitoylglycerol | - |
? | |
| CTP + 1-oleoyl-2-palmitoyl phosphatidic acid | reaction with 1-oleoyl-2-palmitoyl phosphatidic acid at 83.4% of the activity with phosphatidic acid from egg phosphatidylcholine | Escherichia coli | diphosphate + CDP-1-oleoyl-2-palmitoylglycerol | - |
? | |
| CTP + 1-palmitoyl-2-oleoyl phosphatidic acid | reaction with 1-oleoyl-2-palmitoyl phosphatidic acid at 99.7% of the activity with phosphatidic acid from egg phosphatidylcholine | Escherichia coli | diphosphate + CDP-1-palmitoyl-2-oleoylglycerol | - |
? | |
| CTP + phosphatidate | high activity with phosphatidic acid from egg phosphatidylcholine | Escherichia coli | diphosphate + CDPdiacylglycerol | - |
r | |
| CTP + phosphatidate | equilibrium constant is 0.22 | Escherichia coli | diphosphate + CDPdiacylglycerol | - |
r | |
| dCTP + phosphatidate | reaction at the same rate as CTP | Escherichia coli | diphosphate + dCDPdiacylglycerol | - |
? | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.59 | - |
CTP | pH 7.4, 30°C | Escherichia coli | |
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