Cloned (Comment) | Organism |
---|---|
truncated enzyme form | Penicillium chrysogenum |
Protein Variants | Comment | Organism |
---|---|---|
del396-573 | recombinant ATP sulfurylase lacking the C-terminal allosteric domain is monomeric and noncooperative. Mutant enzyme is less heat stable than wild-type enzyme. Wild-type enzyme behaves cooperative at pH 6.5, truncated enzyme form displays normal hyperbolic behavior | Penicillium chrysogenum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3'-phosphoadenosine 5'-phosphosulfate | allosteric, binding of the inhibitor to the catalytic site as well as to the allosteric site of the wild type enzyme acts to decrease the degree of cooperativity | Penicillium chrysogenum | |
5,5'-dithiobis(2-nitrobenzoic acid) | 0.05 mM, rapid decrease in activity of wild-type enzyme (t1/2: 20 s), truncated enzyme del396-573 retains more than 97% of its activity after 30 min | Penicillium chrysogenum | |
adenosine 5'-phosphosulfate | competitive with both substrates | Penicillium chrysogenum | |
diphosphate | noncompetitive with respect to MgATP and sulfate | Penicillium chrysogenum | |
FSO3- | 0.03 mM, 50% inhibition | Penicillium chrysogenum | |
NEM | 0.15 mM, rapid decrease in activity of wild-type enzyme (t1/2: 45 s), truncated enzyme del396-573 retains more than 97% of its activity after 30 min | Penicillium chrysogenum | |
Phenylglyoxal | 3 mM, irreversible inactivation of wild-type enzyme and mutant enzyme del396-573, t1/2: 5 min for both forms | Penicillium chrysogenum | |
thiosulfate | competitive with molybdate and noncompetitive with MgATP | Penicillium chrysogenum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0004 | - |
adenylylsulfate | pH 8.0, 30°C, wild-type enzyme | Penicillium chrysogenum | |
0.0005 | - |
adenylylsulfate | pH 8.0, 30°C, truncated mutant enzyme del396-573 | Penicillium chrysogenum | |
0.0092 | - |
diphosphate | pH 8.0, 30°C, wild-type enzyme | Penicillium chrysogenum | |
0.025 | - |
diphosphate | pH 8.0, 30°C, truncated mutant enzyme del396-573 | Penicillium chrysogenum | |
0.027 | - |
ATP | pH 8.0, 30°C, molybdolysis, truncated mutant enzyme del396-573 | Penicillium chrysogenum | |
0.027 | - |
ATP | pH 8.0, 30°C, molybdolysis, wild-type enzyme | Penicillium chrysogenum | |
0.076 | - |
MoO42- | pH 8.0, 30°C, wild-type enzyme | Penicillium chrysogenum | |
0.21 | - |
ATP | pH 8.0, 30°C, synthesis of adenylylsulfate, wild-type enzyme | Penicillium chrysogenum | |
0.29 | - |
sulfate | pH 8.0, 30°C, wild-type enzyme | Penicillium chrysogenum | |
0.53 | - |
MoO42- | pH 8.0, 30°C, truncated mutant enzyme del396-573 | Penicillium chrysogenum | |
2.6 | - |
ATP | pH 8.0, 30°C, synthesis of adenylylsulfate, truncated mutant enzyme del396-573 | Penicillium chrysogenum | |
3.6 | - |
sulfate | pH 8.0, 30°C, truncated mutant enzyme del396-573 | Penicillium chrysogenum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penicillium chrysogenum | Q12650 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + MoO42- | - |
Penicillium chrysogenum | AMP + adenylylmolybdate | - |
r | |
ATP + sulfate | - |
Penicillium chrysogenum | diphosphate + adenylylsulfate | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | wild-type enzyme | Penicillium chrysogenum |
monomer | 1 * 46000,truncated mutant enzyme del396-573, SDS-PAGE | Penicillium chrysogenum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
t1/2 for mutant enzyme del396-573: 1.5 min | Penicillium chrysogenum |
50 | - |
t1/2 for mutant enzyme del396-573: 0.3 min, wild-type enzyme is stable for more than 2 h | Penicillium chrysogenum |
62 | - |
t1/2 for wild-type enzyme: 1.5 min | Penicillium chrysogenum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.8 | - |
ATP | pH 8.0, 30°C, synthesis of adenylylsulfate, truncated mutant enzyme del396-573 | Penicillium chrysogenum | |
1.8 | - |
sulfate | pH 8.0, 30°C, truncated mutant enzyme del396-573 | Penicillium chrysogenum | |
10.8 | - |
ATP | pH 8.0, 30°C, synthesis of adenylylsulfate, wild-type enzyme | Penicillium chrysogenum | |
10.8 | - |
sulfate | pH 8.0, 30°C, wild-type enzyme | Penicillium chrysogenum | |
13.7 | - |
ATP | pH 8.0, 30°C, molybdolysis, truncated mutant enzyme del396-573 | Penicillium chrysogenum | |
13.7 | - |
MoO42- | pH 8.0, 30°C,truncated mutant enzyme del396-573 | Penicillium chrysogenum | |
24.4 | - |
ATP | pH 8.0, 30°C, molybdolysis, wild-type enzyme | Penicillium chrysogenum | |
24.4 | - |
MoO42- | pH 8.0, 30°C, wild-type enzyme | Penicillium chrysogenum | |
46.6 | - |
adenylylsulfate | pH 8.0, 30°C, truncated mutant enzyme del396-573 | Penicillium chrysogenum | |
46.6 | - |
adenylylsulfate | pH 8.0, 30°C, wild-type enzyme | Penicillium chrysogenum | |
73.3 | - |
diphosphate | pH 8.0, 30°C, truncated mutant enzyme del396-573 | Penicillium chrysogenum | |
73.3 | - |
diphosphate | pH 8.0, 30°C, wild-type enzyme | Penicillium chrysogenum |