KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.045 | - |
ATP | pH 6.0, 40°C | Geobacillus stearothermophilus | |
0.16 | - |
dATP | pH 6.0, 40°C | Geobacillus stearothermophilus | |
0.2 | - |
SO42- | pH 6.0, 40°C | Geobacillus stearothermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | divalent cation required: Mg2+, Mn2+ or Co2+ | Geobacillus stearothermophilus | |
Mg2+ | divalent cation required: Mg2+, Mn2+ or Co2+ | Geobacillus stearothermophilus | |
Mn2+ | divalent cation required: Mg2+, Mn2+ or Co2+ | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
2 * 44000, SDS-PAGE | Geobacillus stearothermophilus |
100000 | - |
gel filtration | Geobacillus stearothermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + sulfate | Geobacillus stearothermophilus | the enzyme catalyzes the first step of sulfate activation | diphosphate + adenylylsulfate | - |
r | |
ATP + sulfate | Geobacillus stearothermophilus NCA 1503 | the enzyme catalyzes the first step of sulfate activation | diphosphate + adenylylsulfate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Geobacillus stearothermophilus NCA 1503 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.04 | - |
- |
Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + sulfate | - |
Geobacillus stearothermophilus | diphosphate + adenylylsulfate | - |
r | |
ATP + sulfate | the enzyme catalyzes the first step of sulfate activation | Geobacillus stearothermophilus | diphosphate + adenylylsulfate | - |
r | |
ATP + sulfate | - |
Geobacillus stearothermophilus NCA 1503 | diphosphate + adenylylsulfate | - |
r | |
ATP + sulfate | the enzyme catalyzes the first step of sulfate activation | Geobacillus stearothermophilus NCA 1503 | diphosphate + adenylylsulfate | - |
r | |
dATP + SO42- | - |
Geobacillus stearothermophilus | diphosphate + deoxyadenylylsulfate | - |
? | |
dATP + SO42- | - |
Geobacillus stearothermophilus NCA 1503 | diphosphate + deoxyadenylylsulfate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 44000, SDS-PAGE | Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
15 min, stable below. 20% loss of activity after 1 h | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 8.7 | - |
Geobacillus stearothermophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.2 | 9.1 | pH 7.2: about 45% of maximal activity, pH 9.1: about 50% of maximal activity | Geobacillus stearothermophilus |