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Literature summary for 2.7.7.4 extracted from

  • Onda, M.; Morimoto, A.; Simoide, A.; Iwata, K.; Nakajima, H.
    Purification and properties of adenosine 5'-triphosphate sulfurylase from the thermophilic bacterium Bacillus stearothermophilus (1996), Biosci. Biotechnol. Biochem., 60, 1740-1742.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.045
-
ATP pH 6.0, 40°C Geobacillus stearothermophilus
0.16
-
dATP pH 6.0, 40°C Geobacillus stearothermophilus
0.2
-
SO42- pH 6.0, 40°C Geobacillus stearothermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ divalent cation required: Mg2+, Mn2+ or Co2+ Geobacillus stearothermophilus
Mg2+ divalent cation required: Mg2+, Mn2+ or Co2+ Geobacillus stearothermophilus
Mn2+ divalent cation required: Mg2+, Mn2+ or Co2+ Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
2 * 44000, SDS-PAGE Geobacillus stearothermophilus
100000
-
gel filtration Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + sulfate Geobacillus stearothermophilus the enzyme catalyzes the first step of sulfate activation diphosphate + adenylylsulfate
-
r
ATP + sulfate Geobacillus stearothermophilus NCA 1503 the enzyme catalyzes the first step of sulfate activation diphosphate + adenylylsulfate
-
r

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-
Geobacillus stearothermophilus NCA 1503
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.04
-
-
Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + sulfate
-
Geobacillus stearothermophilus diphosphate + adenylylsulfate
-
r
ATP + sulfate the enzyme catalyzes the first step of sulfate activation Geobacillus stearothermophilus diphosphate + adenylylsulfate
-
r
ATP + sulfate
-
Geobacillus stearothermophilus NCA 1503 diphosphate + adenylylsulfate
-
r
ATP + sulfate the enzyme catalyzes the first step of sulfate activation Geobacillus stearothermophilus NCA 1503 diphosphate + adenylylsulfate
-
r
dATP + SO42-
-
Geobacillus stearothermophilus diphosphate + deoxyadenylylsulfate
-
?
dATP + SO42-
-
Geobacillus stearothermophilus NCA 1503 diphosphate + deoxyadenylylsulfate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 44000, SDS-PAGE Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
15 min, stable below. 20% loss of activity after 1 h Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 8.7
-
Geobacillus stearothermophilus

pH Range

pH Minimum pH Maximum Comment Organism
7.2 9.1 pH 7.2: about 45% of maximal activity, pH 9.1: about 50% of maximal activity Geobacillus stearothermophilus