Cloned (Comment) | Organism |
---|---|
overexpression in Eacherichia coli | Archaeoglobus fulgidus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
diphosphate | - |
Archaeoglobus fulgidus | |
0.17 | - |
adenosine 5'-phosphosulfate | - |
Archaeoglobus fulgidus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
2 * 57000, SDS-PAGE | Archaeoglobus fulgidus |
150000 | - |
gel filtration | Archaeoglobus fulgidus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + sulfate | Archaeoglobus fulgidus | constitutive enzyme | diphosphate + adenylylsulfate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Archaeoglobus fulgidus | - |
DSM 4304T | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Archaeoglobus fulgidus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.1 | - |
cell extract | Archaeoglobus fulgidus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + sulfate | - |
Archaeoglobus fulgidus | diphosphate + adenylylsulfate | - |
r | |
ATP + sulfate | constitutive enzyme | Archaeoglobus fulgidus | diphosphate + adenylylsulfate | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 57000, SDS-PAGE | Archaeoglobus fulgidus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
wild-type enzyme | Archaeoglobus fulgidus |
100 | - |
recombinant enzyme | Archaeoglobus fulgidus |