Literature summary for 2.7.7.4 extracted from

Seubert, P.A.; Hoang, L.; Renosto, F.; Segel, I.H.
ATP sulfurylase from Penicillium chrysogenum: measurements of the true specific activity of an enzyme subject to potent product inhibition and a reassessment of the kinetic mechanism (1983), Arch. Biochem. Biophys., 225, 679-691.

Search for more literature for 2.7.7.4

Inhibitors

Inhibitors	Comment	Organism	Structure
adenosine 5'-phosphosulfate	potent product inhibition, competitive with both MgATP2- and MoO42- in molybdolysis assay	Penicillium chrysogenum
ClO3-	competitive with SO42- or MoO42-, competitive against MgATP2-	Penicillium chrysogenum
ClO4-	competitive with SO42- or MoO42-, competitive against MgATP2-	Penicillium chrysogenum
FSO3-	competitive with SO42- or MoO42-, competitive against MgATP2-	Penicillium chrysogenum
NO3-	competitive with SO42- or MoO42-, competitive against MgATP2-	Penicillium chrysogenum
S2O32-	dead-end inhibitor, competitive with SO42- or MoO42-, noncompetitive against MgATP2-	Penicillium chrysogenum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.15	-	ATP	pH 8.0, 30°C	Penicillium chrysogenum
0.15	-	ATP	ATP in form of MgATP2-	Penicillium chrysogenum
0.36	-	SO42-	pH 8.0, 30°C	Penicillium chrysogenum

Organism

Organism	UniProt	Comment	Textmining
Penicillium chrysogenum	-	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
ATP + sulfate = diphosphate + adenylyl sulfate	random sequence for the forward reaction with adenylylsulfate release being partially rate limiting	Penicillium chrysogenum	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + sulfate	-	Penicillium chrysogenum	diphosphate + adenylylsulfate	-	r
ATP + sulfate	-	Penicillium chrysogenum	diphosphate + adenylyl sulfate	-	r

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0034	-	FSO3-	pH 8.0, 30°C	Penicillium chrysogenum

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)