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Literature summary for 2.7.7.3 extracted from

  • Edwards, T.E.; Leibly, D.J.; Bhandari, J.; Statnekov, J.B.; Phan, I.; Dieterich, S.H.; Abendroth, J.; Staker, B.L.; Van Voorhis, W.C.; Myler, P.J.; Stewart, L.J.
    Structures of phosphopantetheine adenylyltransferase from Burkholderia pseudomallei (2011), Acta Crystallogr. Sect. F, 67, 1032-1037.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)R3 Rosetta cells Burkholderia pseudomallei

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 0.2 M ammonium sulfate, 0.1 M sodium acetate pH 4.6, 30% (w/v) PEG 2000 Burkholderia pseudomallei

Organism

Organism UniProt Comment Textmining
Burkholderia pseudomallei Q3JW91
-
-

Purification (Commentary)

Purification (Comment) Organism
His Trap column chromatography, and Superdex 200 gel filtration Burkholderia pseudomallei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + pantetheine 4'-phosphate
-
Burkholderia pseudomallei diphosphate + 3'-dephospho-CoA
-
?

Subunits

Subunits Comment Organism
homohexamer x-ray crystallography Burkholderia pseudomallei

Synonyms

Synonyms Comment Organism
phosphopantetheine adenylyltransferase
-
Burkholderia pseudomallei
PPAT
-
Burkholderia pseudomallei

General Information

General Information Comment Organism
metabolism the enzyme catalyzes the fourth of five steps in the coenzyme A biosynthetic pathway Burkholderia pseudomallei