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Literature summary for 2.7.7.2 extracted from
- Substrate specificity and variables affecting efficiency of mammalian flavin adenine dinucleotide synthetase (1989), Biochemistry, 28, 8439-8446.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,5-Dideaza-FMN | - |
Rattus norvegicus |  |
| 1-deaza-FMN | - |
Rattus norvegicus | |
| 2-thio-FMN | - |
Rattus norvegicus | |
| 5'-sulfo-FMN | - |
Rattus norvegicus | |
| 8-hydroxy-7-methyl-FMN | - |
Rattus norvegicus | |
| D-glucose 6-phosphate | - |
Rattus norvegicus | |
| D-lyxose 5-phosphate | - |
Rattus norvegicus | |
| diphosphate | product inhibition | Rattus norvegicus | |
| FAD | - |
Rattus norvegicus | |
| hexyl 6-phosphate | - |
Rattus norvegicus | |
| additional information | no detectable inhibition with 2-[(hydroxyethyl)amino]-FMN, 2-morpholinyl-FMN, 2-(phenylamino)-FMN, 3-methyl-FMN, 3-(carboxymethyl)-FMN, 8-alpha-imidazolyl-FMN, 8-alpha-(N-methylimidazolyl)-FMN, 5'-phosphothionate, DL-glycerol 3-phosphate and propyl 3-phosphate | Rattus norvegicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0048 | - |
7,8-dibromo-FMN | pH 7.1, 37°C, direct assay | Rattus norvegicus | |
| 0.0076 | - |
7-chloro-FMN | pH 7.1, 37°C, indirect assay | Rattus norvegicus | |
| 0.0079 | - |
FMN | pH 7.1, 37°C, indirect assay | Rattus norvegicus | |
| 0.0086 | - |
7-chloro-FMN | pH 7.1, 37°C, direct assay | Rattus norvegicus | |
| 0.0094 | - |
FMN | pH 7.1, 37°C | Rattus norvegicus | |
| 0.0095 | - |
FMN | pH 7.1, 37°C, direct assay | Rattus norvegicus | |
| 0.015 | - |
iso-FMN | pH 7.1, 37°C | Rattus norvegicus | |
| 0.019 | - |
8-chloro-FMN | pH 7.1, 37°C | Rattus norvegicus | |
| 0.082 | - |
7,8-dichloro-FMN | pH 7.1, 37°C, indirect assay | Rattus norvegicus | |
| 0.12 | - |
7,8-dichloro-FMN | pH 7.1, 37°C, direct assay | Rattus norvegicus | |
| 0.25 | - |
4'-butyl-FMN | pH 7.1, 37°C | Rattus norvegicus | |
| 0.94 | - |
7,8-dibromo-FMN | pH 7.1, 37°C, indirect assay | Rattus norvegicus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + FMN | Rattus norvegicus | - |
diphosphate + FAD | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
rat | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0062 | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 4'-butyl-FMN | - |
Rattus norvegicus | diphosphate + 4'-butylflavin adenine dinucleotide | - |
? | |
| ATP + 5'-pentyl-FMN | - |
Rattus norvegicus | diphosphate + 5'-pentylflavin adenine dinucleotide | - |
? | |
| ATP + 7,8-dibromo-FMN | - |
Rattus norvegicus | diphosphate + 7,8-dibromoflavin adenine dinucleotide | - |
? | |
| ATP + 7,8-dichloro-FMN | - |
Rattus norvegicus | diphosphate + 7,8-dichloroflavin adenine dinucleotide | - |
? | |
| ATP + 7-chloro-FMN | - |
Rattus norvegicus | diphosphate + 7-chloroflavin adenine dinucleotide | - |
? | |
| ATP + 8-chloro-FMN | - |
Rattus norvegicus | diphosphate + 8-chloroflavin adenine dinucleotide | - |
? | |
| ATP + FMN | - |
Rattus norvegicus | diphosphate + FAD | - |
ir | |
| ATP + iso-FMN | - |
Rattus norvegicus | diphosphate + isoflavin adenine dinucleotide | - |
? | |
| additional information | if the hydrogen-bonding capacity of the NH group at position 3 is blocked or removed by substitution, FMN analogues do not act as substrates or inhibitors, 3-deaza-FMN, 7,8-didemethyl-8-hydroxy-5-deaza-FMN, 5-methyl-7,8-didemethyl-8-hydroxy-5-deaza-(5-methyl)-FMN, 5'-sulfate-FMN, 5'-deoxy-FMN, 10-(3-chlorobenzyl)-FMN and 10-(hydroxyethyl)-5-deaza-FMN are no substrates | Rattus norvegicus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| flavin adenine dinucleotide synthetase | - |
Rattus norvegicus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
8-hydroxy-7-methyl-FMN | pH 7.1, 37°C | Rattus norvegicus | |
| 0.019 | - |
D-glucose 6-phosphate | pH 7.1, 37°C | Rattus norvegicus | |
| 0.029 | - |
1,5-Dideaza-FMN | pH 7.1, 37°C | Rattus norvegicus | |
| 0.106 | - |
2-thio-FMN | pH 7.1, 37°C | Rattus norvegicus | |
| 0.184 | - |
D-lyxose 5-phosphate | pH 7.1, 37°C | Rattus norvegicus | |
| 0.266 | - |
hexyl 6'-phosphate | pH 7.1, 37°C | Rattus norvegicus | |
| 0.283 | - |
1-deaza-FMN | pH 7.1, 37°C | Rattus norvegicus | |
| 0.291 | - |
5'-sulfo-FMN | pH 7.1, 37°C | Rattus norvegicus | |
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