Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta (DE3) cells | Homo sapiens |
expressed in Escherichia coli Rosetta (DE3) cells | Xenopus tropicalis |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 2% (w/v) tacsimate pH 5.0, 0.15 M sodium citrate tribasic dehydrate pH 5.6 and 10% (w/v) PEG 3350 at 10°C | Xenopus tropicalis |
Protein Variants | Comment | Organism |
---|---|---|
D124A | the mutant shows strongly reduced activity compared to the wild type enzyme | Homo sapiens |
D126A | the mutant shows strongly reduced activity compared to the wild type enzyme | Homo sapiens |
E200A | the mutant shows strongly reduced activity compared to the wild type enzyme | Homo sapiens |
G107A | the mutant shows strongly reduced activity compared to the wild type enzyme | Homo sapiens |
S108A | the mutant shows strongly reduced activity compared to the wild type enzyme | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Homo sapiens | 5737 | - |
cytoplasm | - |
Xenopus tropicalis | 5737 | - |
nucleus | - |
Homo sapiens | 5634 | - |
nucleus | - |
Xenopus tropicalis | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 4 mM used in assay conditions. When Mg2+ is replaced by Mn2+, the protein becomes more potent in catalyzing polyadenylation | Homo sapiens | |
Mg2+ | 4 mM used in assay conditions. When Mg2+ is replaced by Mn2+, the protein becomes more potent in catalyzing polyadenylation | Xenopus tropicalis | |
Mn2+ | 4 mM used in assay conditions | Homo sapiens | |
Mn2+ | 4 mM used in assay conditions | Xenopus tropicalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + RNAn | Homo sapiens | - |
diphosphate + RNAn+1 | - |
? | |
ATP + RNAn | Xenopus tropicalis | - |
diphosphate + RNAn+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q96A09 | - |
- |
Xenopus tropicalis | F7E7M3 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superdex 200 gel filtration | Homo sapiens |
Ni-NTA column chromatography and Superdex 200 gel filtration | Xenopus tropicalis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | - |
Homo sapiens | - |
embryonic stem cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + RNA (A)15 | - |
Homo sapiens | diphosphate + RNA(A)16 | - |
? | |
ATP + RNA (A)15 | - |
Xenopus tropicalis | diphosphate + RNA(A)16 | - |
? | |
ATP + RNAn | - |
Homo sapiens | diphosphate + RNAn+1 | - |
? | |
ATP + RNAn | - |
Xenopus tropicalis | diphosphate + RNAn+1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FAM46B | - |
Homo sapiens |
FAM46B | - |
Xenopus tropicalis |
PAP | - |
Homo sapiens |
PAP | - |
Xenopus tropicalis |
poly(A) polymerase | - |
Homo sapiens |
poly(A) polymerase | - |
Xenopus tropicalis |
Organism | Comment | Expression |
---|---|---|
Homo sapiens | the enzyme is highly expressed in human pre-implantation embryos and pluripotent stem cells | up |
General Information | Comment | Organism |
---|---|---|
malfunction | enzyme knock-out is lethal. Knock-down of enzyme induces apoptosis and restricts protein synthesis | Homo sapiens |
physiological function | the enzyme is indispensable for the viability of human embryonic stem cells | Homo sapiens |