Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.19 extracted from

  • Zhelkovsky, A.; Helmling, S.; Bohm, A.; Moore, C.
    Mutations in the middle domain of yeast poly(A) polymerase affect interactions with RNA but not ATP (2004), RNA, 10, 558-564.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.19

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
K215A 74- and 56-fold increase in the Km for oligo(A)14 in comparison with wild-type enzyme, 2.8-3.6-fold decrease in Vmax Saccharomyces cerevisiae
N226A 74- and 56-fold increase in the Km for oligo(A)14 in comparison with wild-type enzyme Saccharomyces cerevisiae
Y224F 30-fold increase in the Km for oligo(A)14 in comparison with wild-type enzyme, 2.8-3.6-fold decrease in Vmax Saccharomyces cerevisiae
Y224S 12-fold increase in the Km for oligo(A)14 in comparison with wild-type enzyme, 2.8-3.6-fold decrease in Vmax Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
additional information Fip1, a component of yeast polyadenylation factor I, has an inhibitory effect on the enzyme activity because it competes with RNA for access to the C-RBS region Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0005
-
 oligo(A)14 wild-type PAP, incubation at 30 °C Saccharomyces cerevisiae
0.006
-
 oligo(A)14 mutant Y224S Saccharomyces cerevisiae
0.015
-
 oligo(A)14 mutant Y224F Saccharomyces cerevisiae
0.028
-
 oligo(A)14 mutant N226A Saccharomyces cerevisiae
0.031
-
 ATP mutant Y224S Saccharomyces cerevisiae
0.037
-
 oligo(A)14 mutant K215A Saccharomyces cerevisiae
0.04
-
 ATP wild-type PAP Saccharomyces cerevisiae
0.06
-
 ATP mutant Y224F Saccharomyces cerevisiae
0.062
-
 ATP mutant N226A Saccharomyces cerevisiae
0.096
-
 ATP mutant K215A Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 recombinant wild-type and mutant Pap1 were expressed, using the T7 expression system
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + oligo(A)14
-
 Saccharomyces cerevisiae diphosphate + oligo(A)15
-
 ?
ATP + RNAn
-
 Saccharomyces cerevisiae diphosphate + RNAn+1
-
 ?

Synonyms

Synonyms Comment Organism
poly(A) polymerase
-
 Saccharomyces cerevisiae