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Literature summary for 2.7.7.19 extracted from

  • Das Gupta, J.; Li, Q.; Thomson, A.B.; Hunt, A.G.
    Characterization of a novel plant poly(A) polymerase (1995), Plant Sci., 110, 215-226.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
3'-dATP
-
Pisum sativum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.039
-
ATP 30°C, pH 8.0 Pisum sativum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Zea mays
Mg2+ more active in presence of Mg2+ than Mn2+ Pisum sativum

Organism

Organism UniProt Comment Textmining
Brassica juncea
-
-
-
Pisum sativum
-
-
-
Zea mays
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pisum sativum
-
Zea mays
-
Brassica juncea

Renatured (Commentary)

Renatured (Comment) Organism
chromatography on MonoQ results in three fractions with little activity, one of them containing a 43000 polypeptide, the others RNAs, mixtures of them result in substantial enzymic activity Pisum sativum
chromatography on MonoQ results in three fractions with little activity, one of them containing a 43000 polypeptide, the others RNAs, mixtures of them result in substantial enzymic activity Zea mays
chromatography on MonoQ results in three fractions with little activity, one of them containing a 43000 polypeptide, the others RNAs, mixtures of them result in substantial enzymic activity Brassica juncea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + RNA
-
Pisum sativum diphosphate + RNA(A)n
-
?
ATP + RNA
-
Zea mays diphosphate + RNA(A)n
-
?
ATP + RNA
-
Brassica juncea diphosphate + RNA(A)n
-
?