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Literature summary for 2.7.7.19 extracted from

  • Sastre, L.; Sebastian, J.
    Purification and properties of a polyadenylate polymerase from Artemia dormant embryos (1981), Biochim. Biophys. Acta, 661, 54-62.
No PubMed abstract available
Search for more literature for 2.7.7.19

Inhibitors

Inhibitors Comment Organism Structure
ATP above 0.5 mM Artemia sp.
K+ 80 mM: 50% inhibition; KCl Artemia sp.
NH4+ 50 mM: 50% inhibition; ammonium sulfate Artemia sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 ATP 30°C, pH 8.5 Artemia sp.
0.06
-
 dATP 30°C, pH 8.5 Artemia sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ required Artemia sp.
Mn2+ optimal concentration: 2-4 mM Artemia sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 gel filtration Artemia sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + RNA Artemia sp. processing and activation of stored mRNAs after resumption of development ?
-
 ?

Organism

Organism UniProt Comment Textmining
Artemia sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Artemia sp.

Source Tissue

Source Tissue Comment Organism Textmining
embryo encysted, dormant Artemia sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Artemia sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + RNA primer required Artemia sp. diphosphate + RNA(A)n length of the poly(A) tail is dependent on incubation time and RNA primer concentration ?
ATP + RNA no specificity for the 3'-terminal nucleotides Artemia sp. diphosphate + RNA(A)n length of the poly(A) tail is dependent on incubation time and RNA primer concentration ?
ATP + RNA processing and activation of stored mRNAs after resumption of development Artemia sp. ?
-
 ?
dATP + RNA 15% of the activity with ATP Artemia sp. diphosphate + ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 5 min, stable Artemia sp.
45
-
 5 min, 50% loss of activity Artemia sp.
50
-
 5 min, complete inactivation Artemia sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5
-
 Artemia sp.

pH Range

pH Minimum pH Maximum Comment Organism
7 8.5 pH 7: about 40% of maximum activity, pH 8-8.5: maximum activity Artemia sp.