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Literature summary for 2.7.7.19 extracted from

  • Saluja, D.; Mathur, M.; Sachar, R.C.
    Purification of poly(A) polymerase from mung bean hypocotyls: subunit structure, molecular properties and characterization of the reaction product (1989), Plant Sci., 60, 27-38.
No PubMed abstract available
Search for more literature for 2.7.7.19

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol required Vigna radiata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.047
-
 ATP pH 8.0, 37°C Vigna radiata

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ required Vigna radiata
Mn2+ optimal concentration: 2 mM Vigna radiata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 4 * 30000, SDS-PAGE Vigna radiata
120000
-
 gel filtration Vigna radiata

Organism

Organism UniProt Comment Textmining
Vigna radiata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Vigna radiata

Source Tissue

Source Tissue Comment Organism Textmining
hypocotyl
-
 Vigna radiata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Vigna radiata

Storage Stability

Storage Stability Organism
-70°C, stable for several weeks Vigna radiata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + RNA primer required Vigna radiata diphosphate + RNA(A)n
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 30000, SDS-PAGE Vigna radiata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Vigna radiata

pH Range

pH Minimum pH Maximum Comment Organism
7 9 about 65% of maximum activity at pH 7.0 and pH 9.0 Vigna radiata